Mesenchymal Stem Cell Therapy for Multiple Sclerosis
-
M Mohyeddin Bonab
,
S Yazdanbakhsh
,
K Alimoghaddom
,
A Ghavamzadeh
,
F Hooshmand
,
J Lotfi
,
F Talebian
,
B Nikbin
Abstract
Human mesenchymal stem cell (MSC) can be isolated from bone marrow (BM) and differentiated into multiple lineages. These properties make them promising tools in cell and gene therapy. Up to now, no definite therapeutic intervention for late stages of multiple sclerosis (MS) has been found. We decided to inject MS patients with autologous expanded MSC. Five patients participated in this ongoing study. Patients were injected intrathecally with the culture expanded BM MSCs. Patients were followed monthly for their clinical status and every 3 months re¬garding their magnetic resonance imaging. During 7 months follow up, one patient improved 1.5 EDSS, two patients improved by 1 and 2 scores, and two others remained unchanged till now. The first MRI findings of patients showed no change. We can claim that the injection of expanded MSC is a safe procedure. Three patients showed some de¬gree of improvement and the other two had no progression. Patients should be followed for at least one year and a larger sample is r quired in order to draw a definite conclusion.
Muraro PA et al. Immunological questions on hemato- poietic stem cell transplantation for multiple sclerosis. Bone marrow transplantation 32, S41-S44: 2003.
Pereira RF at al. 1998. Marrow stromal cells as a source of progenitor cells for nonhematopoietic tisssues in trangenic mice with a phenotype of osteogenesis im- perfecta. Proc Natl.Acad.Sci.USA 95:1142-1147.
Le Blanc K. 2002. Mesenchymal stem cells. Basic sci- ence and future clinical use. Lakartidningen . Mar 21; 99(12): 1318-21,1324.
Lee ST et al. 2002Treatment of high-risk acute mye- logenous leukaemia by myeloablative chemoradiotherapy followed by co-infusion of cell-depleted haematopoietic stem cells and culture-expanded marrow mesenchymal stem cells from a related donor with one fully mis- matched human leucocyte antigen haplotype. Br J Haematol, Sep; 118(4): 1128-31.
David steenblock.2003. Alternative therapies for Mul- tiple sclorosis. Curr Opin Neurol 16(3):299-305.
Ben- Hur T et al.2003. transplanted multipotential neu- ral precursor cells migrate into the inflamed white matter in response to experimental autoimmune encephalomye- litis. Glia 41 (1):73-80.
Burt RK et al. 1998. Treatment of autoimmune disease by intense immunosuppressive conditioning and autolo- gous hematopoietic stem cell transplantation . Blood 92 (10):3505-14.
Marmont AM.2000.New horizons in the treatment of autoimmune diseases; immunoablation and stem cell transplantation. Annu Rev Med 51:115-134;.
Blanco Yet al.2005. Autologous Hematopoetic stem cell transplantation for Multiple Sclerosis. Lancet Neu- rology, 4:54-63.
Smith PM, Franklin RJ. 2001. The effect of immuno- suppression protocols on spontaneous CNS remyelination following toxin-induced demyelination. J Neuroimmunol 119:261-268.
Prockop, D. 1997. Marrow stromal cells for nonhe- matopoietic tissues. Science; 276:71-74.
Priller J et al. 2001.Neogenesis of cerebellar purkinje neurons from gene- marked bone marrow cells in vivo. J.Cell Biol.; 26:733-738.
Kopen GC, Prockop DJ. and Phinney DG.1999.Marrow stromal cells migrate throughout the forebrain and cerebellum and they differentiate into as- trocytes after injection into neonatal mouse brains. Proc.Natl.Acad.sci.USA,96:10711-10716.
Jian-Yong Min et al. 2002. Significant improvement of heart function by cotransplantation of human mesen- chymal stem cells and fetal cardiomyocytes in postinfarc- ted pigs. The Annals of thoracic surgery 74(5):1568-75.
Bartholomew A et al.2002.Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. Exp Hematol. Jan; 30(1):42-8.
Djouad F et al.2003. Immunosuppressive effect of mesenchymal stem cells favors tumor growth in alloge- neic animals. Blood 4:1193 (Abstract)
Martin R, McFarland HF. Immunology of multiple sclerosis ex- perimental allergic encephalomyelitis. 1997. In: Raine CS, McFarland HF, Tourtellotte WW(eds), Multiple scle- rosis: clinical and pathogenetic basis chapman & Hall: London, PP221-242.
Goebels N,Hofstetter H, Schmidt S, Brunner C,Wekerle H ,Hohlfeld R. 2000. Repertoire dynamics of autoreactive T cells in multiple sclerosis patients and healthy subjects-Epitope spreading versus clonal persis- tance. Brain 123: 508-518.
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