Comparative Evaluation of Biochemical and Hematological Parameters of Pre-Storage Leukoreduction during RBC Storage

  • Behrooz Ghezelbash Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  • Azita Azarkeivan Pediatric Hematology Oncology, Iranian Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Thalassemia Clinic, Tehran, Iran
  • Ali Akbar Pourfathollah Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  • Mohammadreza Deyhim Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Biochemistry Department, Tehran, Iran
  • Esmerdis Hajati Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Flow Cytometry Department, Tehran, Iran
  • Alireza Goodarzi Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Keywords: leuko-reduction RBC, Blood transfusion, RBC storage, RBC storage lesion

Abstract

Background: Some of the red cell storage lesions (RCSLs) take place during red blood cell (RBC) storage and may reduce the function of these cells dramatically, which mostly caused by residual leucocytes in blood components. This study was planned to observe the biochemical and hematological changes in pre-storage leukoreduced RBC (LR-RBC) compared with unfiltered RBC during in vitro storage. Materials and Methods: Ten unit RBCs were collected, processed and stored according to Iranian standard operating procedure (SOP) of Iranian Blood Transfusion Organization (IBTO). Every unit was split into two equal parts, unfiltered RBC and LR-RBC. Samples were collected and tested on weeks of storage. Biochemical parameters such as lactate dehydrogenase (LDH), lactate concentration and glucose-6-phosphate dehydrogenase (G6PD) enzyme activity were measured by auto-analyzer. In addition, hematology analyzer was used to monitor the change of RBC indices such as (MCV), (MCH) and (MCHC).Results: In this study, both groups showed progressive increase of LDH and lactate levels, and also G6PD activity decreased during storage. Mean of LDH and lactate in unfiltered RBC was significantly increased compared with LR-RBC during all days of storage (p< 0.05). There was statically significant decrease in the G6PD enzyme activity between the two groups and weeks of storage (p< 0.05). However, the RBC indices remained within the expected levels in both groups.Conclusion: LR-RBC and RBC both exhibited RCSL during storage, but LR-RBC is effective in reducing Red cell storage lesion (RCSL) and also improves the quality of stored red blood cells.

References

Dzik S, Aubuchon J, Jeffries L, et al. Leucocyte reduction of blood components: public policy and new technology. Transfus Med Rev. 2000; 114(1): 34–52.

Nagura Y, Tsuno NH, Tanaka M, et al. The effect of pre-storage whole-blood leucocyte reduction on cytokines/chemokines levels in autologous CPDA-1 whole blood. Transfus Apher Sci. 2013; 49(2): 223–230.

Napier A, Chapman J F, Forman K, et al. Guidelines on the clinical use of leucocyte-depleted blood components. Transfusion Medicine 1998; 8(1): 59–71.

Peters AL, Bruggen RV, de Korte D, et al. Glucose-6-phosphate dehydrogenase activity decreases during storage of leucoreduced red blood cells. Transfusion. 2016; 56(2): 427–32.

Miller JP, Mintz PD. The use of leukocyte-reduced blood components. Hematol Oncol Clin North Am. 1995; 9(1): 69-90.

Peters AL, Van Noorden CJ. Glucose-6-phosphate dehydrogenase deficiency and malaria: cytochemical detection of heterozygous G6PD deficiency in women. J Histochem Cytochem. 2009; 57(11): 1003-11.

Radovan U, Bronislav K, Milan A. Biochemical changes in the patient’s plasma after red blood cell transfusion. Signa Vitae 2011; 6(2): 64 -71.

Lippi G, Salvagno LG, Montagnana M, et al. Influence of hemolysis on routine clinical chemistry testing. Clin Chem Lab Med. 2006; 44(3): 311-6.

Gacko M, Radziwon P, Kowalewski R, et al. Some components of oxidative-antioxidative system in human blood plasma and serum. Rocz Akad Med Bialymst. 2004; 49 Suppl1: 190–191.

D’Alessandro A, D’Amici G, Vaglio S, et al. Time-course investigation of SAGM-stored leucocyte-filtered red blood cell concentrates: from metabolism to proteomics. Haematologica. 2012; 97(1): 107–15.

Verma M, Dahiya K, Malik D, et al. Effect of Blood Storage on Complete Biochemistry. J Blood Disord Transfus. 2015; 6(6):1-4.

Latham JT JR, Bove JR, Weirich FL. Chemical and hematologic changes in stored CPDA-1 blood. Transfusion. 1982; 22(2):158-9.

Bailey DN, Bove JR. Chemical and Hematological Changes in Stored CPD Blood. Transfusion. 1975; 15(3): 244-9.

Nedzi M, Chabowska AM, Rogowska A, et al. Leucoreduction helps to preserve activity of antioxidant barrier enzymes in stored red blood cell concentrates. Vox Sang. 2016; 110(2): 126–133.

Pertinhez TA, Casali E, Lindner L, et al. Biochemical assessment of red blood cells during storage by (1)H nuclear magnetic resonance spectroscopy. Identification of a biomarker of their level of protection against oxidative stress. Blood Transfus. 2014; 12(4): 548–56.

Castro O, Hoque M, Brown BD. Pulmonary hypertension in sickle cell disease: cardiac catheterization results and survival. Blood. 2003; 101(4): 1257-61.

Silliman CC, Thurman GW, Ambruso DR. Stored blood components contain agents that prime the neutrophil NADPH oxidase through the plateletactivating- factor receptor. Vox Sang. 1992; 63(2):133–6.

Sonker A, Dubey A, Chaudhary R. Evaluation of a Red Cell Leucofilter Performance and Effect of Buffy Coat Removal on Filtration Efficiency and Post Filtration Storage. Indian J Hematol Blood Transfus. 2014; 30(4): 321–327.

Muller-Steinhardt M, Janetzko K, Kirchner H, et al. Effect of whole blood preparation and leucocyte filtration on storage of erythrocyte concentrates over 42 days. Beitr Infusionsther Transfusionsmed. 1997; 34:53–7.

Ufelle SA, Neboh EE, Ocheni S, et al. The activity of glucose-6-phosphate dehydrogenase (G6PD) in stored blood. Orient Journal of Medicine. 2014; 26(3-4): 94-98.

Richard OF, Jeffrey Jh, Jeanne EH, et al. Frequency of

glucose-6-phosphate dehydrogenase-deficient red blood cell units in a metropolitan transfusion service. Transfusion. 2013; 53(3):606-611.

Rocchigiani M, Pescaglini M, Sestini S, et al. Density increase and ageing of erythrocytes in stored blood. J Int Med Res. 1989; 17(5):461-6.

Nogueira D, Rocha S, Abreu E, et al. Biochemical and cellular changes in leucocyte-depleted red blood cells stored for transfusion. Transfus Med Hemother. 2015; 42(1): 46-51.

Adias TC, Moore-Igwe B, Jeremiah ZA. Storage Related Haematological and Biochemical Changes of CPDA-1 Whole Blood in a Resource Limited Setting. J Blood Disorders Transf. 2012; 3(3): 124.

Published
2018-01-05
How to Cite
1.
Ghezelbash B, Azarkeivan A, Pourfathollah AA, Deyhim M, Hajati E, Goodarzi A. Comparative Evaluation of Biochemical and Hematological Parameters of Pre-Storage Leukoreduction during RBC Storage. ijhoscr. 12(1):35-2.
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Original Article(s)