International Journal of Hematology-Oncology and Stem Cell Research 2018. 12(3):229-233.

Concise Review on Clinical Applications of Conditioned Medium Derived from Human Umbilical Cord-Mesenchymal Stem Cells (UC-MSCs)
Sushmitha Sriramulu, Antara Banerjee, Rosa Di Liddo, Ganesan Jothimani, Madhumala Gopinath, Ramachandran Murugesan, Francesco Marotta, Surajit Pathak


In recent years, mesenchymal stem cells have provoked much attentiveness in the field of regenerative medicine because of their differentiation potential and the capability to facilitate tissue repair via the emancipation of biologically active molecules. They have gained interest because of their distinctive curative properties. Mesenchymal stem cells are isolated from the Wharton’s jelly part of umbilical cord possessing higher proliferation capacity, immunomodulatory activity, plasticity, as well as self-renewal capacity than the mesenchymal stem cells from various origins, and it is considered to be the best resource for allogeneic transplantation. The isolated umbilical cord-derived mesenchymal stem cells are cultured in the Dulbecco’s Modified Eagle’s Medium, and thereby it begins to release soluble factors into the medium during the period of culture which is termed as conditioned medium. This conditioned media has both differentiation capacity and therapeutic functions. Thus, it can be able to differentiate the cells into different lineages and the paracrine effect of these cells helps in replacement of the damaged cells. This medium may accord to optimization of diagnostic and prognostic systems as well as the generation of novel and targeted therapeutic perspectives.


Umbilical cord, Stem cells, Conditioned medium, Therapeutics, Isolation

Full Text:



Ali F, Taresh S, Al-Nuzaily M, et al. Stem cells differentiation and probing their therapeutic applications in hematological disorders: a critical review. Eur Rev Med Pharmacol Sci. 2016; 20(20): 4390-4400.

Mohammadi S, Nikbakht M, Mohammadi AM, et al. Human platelet lysate as a xeno free alternative of fetal bovine serum for the in vitro expansion of human mesenchymal stromal cells. Int J Hematol Oncol Stem Cell Res. 2016; 10(3): 161-71.

Szepesi Á, Matula Z, Szigeti A, et al. In Vitro Characterization of Human Mesenchymal Stem Cells Isolated from Different Tissues with a Potential to Promote Complex Bone Regeneration. Stem Cells Int. 2016; 2016: 3595941.

Viganò M, Sansone V, d'Agostino MC, et al. Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders. J Orthop Surg Res. 2016; 11(1):163.

Girdlestone J. Mesenchymal stromal cells with enhanced therapeutic properties. Immunotherapy. 2016; 8(12): 1405–1416.

Han KH, Kim AK, Kim DI. Therapeutic Potential of Human Mesenchymal Stem Cells for Treating Ischemic Limb Diseases. Int J Stem Cells. 2016; 9(2): 163-168.

Nagamura-Inoue T, He H. Umbilical cord-derived mesenchymal stem cells: Their advantages and potential clinical utility. World J Stem Cells. 2014; 6(2): 195-202.

Shen C, Lie P, Miao T, et al. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Mol Med Rep. 2015; 12(1): 20-30.

Linero I, Chaparro O. Paracrine effect of mesenchymal stem cells derived from human adipose tissue in bone regeneration. PLOS One. 2014; 9(9): e107001.

Li M, Luan F, Zhao Y, et al. Mesenchymal Stem cell-conditioned medium accelerates wound healing with fewer scars. Int Wound J. 2017; 14(1):64-73.

Park CM, Kim MJ, Kim SM, et al. Umbilical cord mesenchymal stem cell-conditioned media prevent muscle atrophy by suppressing muscle atrophy- related proteins and ROS generation. In Vitro Cell Dev Biol Anim. 2016; 52(1): 68-76.

Kim MJ, Kim ZH, Kim SM, et al. Conditioned medium derived from umbilical cord mesenchymal stem cells regenerates atrophied muscles. Tissue Cell. 2016; 48(5):533-43.

Pawitan JA. Prospect of stem cell conditioned medium in regenerative medicine. Biomed Res Int. 2014; 2014: 965849.

Arutyunyan I, Fatkhudinov T, Kananykhina E, et al. Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study. Stem cell Res Ther. 2016; 7:46.

Liu Q, Luo Z, He S, et al. Conditioned serum-free medium from umbilical cord mesenchymal stem cells has anti-photoaging properties. Biotechnol Lett. 2013; 35(10): 1707-14.


  • There are currently no refbacks.

Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.