Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 18 2 2024 04 16 110 116 EN Zahra Naderipour Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Farzane Behnezhad Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Javad Charostad Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Mohsen Nakhaie Gastroenterology and Hepatology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Nadieh Baniasadi Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran Yaser Ghelmani Department of Internal Medicine, Clinical Research Development Center of Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Fateme Akhavan Tafti 1) Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 2) Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Yazd Blood Transfusion Center, Yazd, Iran Azam Dehghani Quantitative and Systems Biology, University of California Merced, 5200 North Lake Rd., Merced, CA, 95343, USA Akram Astani 1) Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2) Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran 2022 04 21 2024 02 17 Background: Torque teno virus (TTV) is a globally prevalent virus in humans, yet comprehensive knowledge about its prevalence, predominant transmission routes, and pathogenesis remains limited. This study aimed to assess the frequency of TTV infection among healthy blood donors in Yazd, Iran. Materials and Methods: A total of 236 healthy blood donors, devoid of HIV/HBV/HCV infection markers, participated in the study from 2015 to 2016. Nested Polymerase Chain Reaction (PCR) utilizing a set of oligo primers for the 5΄- UTR region was employed to detect TTV DNA in serum samples. Results: The TTV genome was identified in 161 out of 236 (61.2%) healthy blood donors. The mean age for men and women was 43 and 57 years, respectively. Of the participants, 156 were male, and 107 were female. Donor age exhibited a significant association with virus presence (P=0.007); however, gender did not show a statistically significant association with the frequency of TTV infection in healthy blood donors (P=0.3). Conclusion: The study revealed a notably high frequency of the Torque teno virus in Yazd province, aligning with similar findings globally. Further investigations are warranted to elucidate the clinical implications of the virus in the healthy population. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1828 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1828/1023