Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 18 2 2024 04 16 117 122 Somayeh Mansournejad Department of Biology, Medicinal Plants, Health and Food Safety Research Center, Borujerd Branch, Islamic Azad University, Borujerd, Iran Mohammad Mehrabi Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran Reza Yari Department of Biology, Medicinal Plants, Health and Food Safety Research Center, Borujerd Branch, Islamic Azad University, Borujerd, Iran Mahshid Saleh Wisconsin National Primate Research Center, University of Wisconsin Graduate School, Madison, WI, USA 2022 06 10 2023 11 15 Background: miR-29c-3p manages a set of genes involved in regenerative medicine, and It seems that hyperglycemia in diabetic patients influences the power of stem cells to tissue regeneration the difficulties of diabetes by affecting the expression miR-29c-3p in mesenchymal stem cells. The study aims to analyze the effect of various glucose concentrations on the miR-29c-3p expression in mesenchymal stem cells. Materials and Methods: After receiving donated mesenchymal stem cells from Tarbiat Modares University, these cells were cultivated in a DMEM culture medium, including three different concentrations of glucose 250, 140, and 100 mg/dl. RNA was extracted from these cells after 72 hours, the Real-Time PCR technique assessed the expression of miR-29c-3p, and the results were analyzed by REST software. Results: miR-29c-3p expression in cells at concentrations of 140 and 250 mg/dL compared to typical situations (100 mg/dl) was significantly decreased (P˂0.05), which declined at a concentration of 250 mg/dl was more. Conclusion: Reduced miR-29c-3p expression in mesenchymal stem cells in chronic and mild diabetic situations demonstrated that diabetes might be one of the significant reasons for mesenchymal stem cells' reduced ability to repair tissue damage. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1860 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1860/1024