Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 4 2 2010 06 15 14 18 Zohreh Saltanatpouri Department of Mycology and Parasitology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran Tahereh Shokohi Department of Mycology and Parasitology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Bagher Hashemi Soteh Department of Biochemistry and Genetics, Sari Medical School, Mazandaran University of Medical Sciences, Tehran, Iran Mohammad Taghi Hedayati Department of Mycology and Parasitology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran 2015 10 01 Introduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly. Materials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done. Results: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans.  The results were confirmed by sequencing and RAPD-PCR. Conclusion:PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/243 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/243/236