Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 6 2 2012 06 15 22 26 Majid Mossahebimohammadi Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran Saeid Kaviani Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran Mina Soufizomorrod Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran Masoud Soleimani Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran Saeid Abroun Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran Gholamreza Khamisipour Department of Allied Medicine, Bushehr University of medical sciences, Bushehr, Iran Najmaldin Saki Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran 2015 10 01 Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/331 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/331/317