Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 7 1 2013 03 15 8 12 Mina Soufizomorrod Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran. Masoud Soleimani Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran. Abbas Hajifathali Department of Medical Oncology and Hematology, Taleghani Hospital, Shahid Beheshti University (MC), Tehran, Iran. Majid Mossahebi Mohammadi Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran. Saeed Abroun Department of Hematology and Blood banking, Tarbiat Modares University, Tehran, Iran. 2015 10 14 Umbilical cord Blood (UCB) as a source of Hematopoietic Stem/Progenitor cells (HSPCs) used for Umbilical cord blood transplantation (UCBT). The main obstacle in application of this source as an appropriate source of HSPCs is low volume of this product. So ex vivo expansion of these cells in a microenvironment which mimic body condition is important. In current study we designed biocompatible microwells in which collagene type I is coated by softlitography method. Our findings designated that in 3-Dimensional (3D) microenvironment CD133(+) UCB derived HSC expanded significantly compared to 2-Dimensional (2D) microenvironment. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/465 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/465/332