Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 1 4 Hamid Choobineh Department of Medical Laboratory Sciences, Faculty of Para- Medicine, Tehran University of Medical Sciences SJ Dehghani Department of Medical Laboratory Sciences, Faculty of Para- Medicine, Tehran University of Medical Sciences Shaban Alizadeh Department of Hematology and Blood Banking, Faculty of Para- Medicine, Tehran University of Medical Sciences V Ghobadi Dana Department of Pediatrics, Faculty of Medicine, Tehran University of Medical Sciences N Saiepour Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences R Meshkani Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences N Einollahi Department of Medical Laboratory Sciences, Faculty of Para- Medicine, Tehran University of Medical Sciences 2015 10 01 Introduction: Leptin is an adipocyte-derived hormone. Exogenous leptin allows the recovery of the reproductive function. In humans, leptin correlates positively with the body mass index (BMI). The aim of this study was to investigate the association of leptin with the toxic effects of iron overload. Methods: 219 Major Beta thalassemic patients (119 men, 100 women) and 137 non thalassemic individuals (86 men, 51 women) were investigated on the basis of a case control study. Data was gathered from six hospitals related to Tehran University during a seven month petiod (July 2006– January 2007). Blood samples of all major beta thalassemic patients who were admitted to these hospitals for recurrent blood transfusion were collected. Non thalassemic individuals were selected from outpatients who without significant medical problems, had come to these hospitals and had extra blood sample. The similarities of the two groups in age, gender and BMI also were considered. Results: The serum leptin level median was 5.00 (interquartile range: 6.50) for major beta thalassemic patients and 6.10 (interquartile range: 7.00) for healthy individuals. Serum leptin level was significantly lower in thalassemic patients (P value <0.001). Major beta thalassemic men had significantly lower leptin level (median, interquartile range: 2.90, 3.60) than major beta thalassemic women (median, interquartile range: 6.45, 16.02; P value <0.001). Conclusions: This study confirmed that the adipocytes of major beta thalassemic patients are unable to maintain adequate leptin production. These results suggest that adipose tissue dysfunction can be considered one of the endocrinopathies affecting major beta thalassemic patients. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/223 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/223/216

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 5 13 MR Baghaban Eslaminejad Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran H Nazarian Department of Stem Cell and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran M Shariati Department of Periodontics, Dental Scool of Shahid Beheshti University, Tehran, Iran S Vahabi Department of Periodontics, Dental Scool of Shahid Beheshti University, Tehran, Iran 2015 10 01 Introduction: Dental pulp-derived mesenchymal stem cells (MSCs) have emerged as a promising tool for use in regenerative medicine. The in vitro growth kinetic and culture requirement of the cells derived from human dental pulp, which is the subject of this present study, is poorly described. Materials and Methods: Stem cells were derived from human third molar and then characterized. The in vitro growth kinetics of the cells was examined by colonogenic assay and a determination of the population doubling number (PDN). Finally, the culture conditions were optimized for pulp stem cell maximum proliferation. Results: Propagated dental pulp cells tended to differentiate into odontoblast, osteoblast, adipose and cartilage cells. Typically surface antigens were expressed as mesenchymal stem cells. The cells tended to be very proliferative with a PDN value of about 11. The colonogenic efficiency was about 60% and an average colony size was about 10.75±1.58 mm2. The best culture condition for enhanced proliferation was achieved when the cells were seeded at 100 cells/cm2 in a the presence of 20% FBS in a medium (P<0.05). Conclusion: Taken together, the optimal culture conditions for human dental pulp-derived MSCs were determined. This information is helpful with respect to cell in vitro propagation which is greatly needed prior to their transplantation. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/224 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/224/217

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 14 20 Amir Hossein Emami Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Ramin Shekarriz Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran A Meysamie Community Medicine Department, Faculty of Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Ramazanali Sharifian Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran R Safaei Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Ghlamreza Toogheh Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran Manouchehr Keihani Hematology-Oncology Department, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran R Shekarriz Community Medicine Department, Faculty of Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2015 10 01 Introduction: About half of acute myeloid leukemia (AML) adult patients have no cytogenetic abnormalities as a main determinant of complete remission after treatment, so other markers are needed such as FLT3-ITD (Fms-like Tyrosine kinase3-internal tandem duplication) mutations in patients with normal karyotype. The objective of this study was assessing the frequency of FLT3-ITD mutations and its relation with complete remission in different FAB (French- American- British) and cytogenetic subgroups of AML patients who had been hospitalized at Tehran Imam Khomeini Hospital, hematology ward. Methods: The current study, was a cross sectional descriptive study which was performed during the years 2007-2008. Population frame were consecutive patients whose diseases were confirmed and who had been hospitalizedin Tehran Imam Khomeini Hospital, hematology ward. Contemporary, flowcytometry, cytogenetic and chromosomal studies were performed for the cytogenetic subgroup assessment and to investigate the presence of FLT3-ITD mutation. Finally, complete remission achievement after induction chemotherapy were assessed. The obtained data was entered onto the information forms and analyzed by statistical tests. Results: Out of 40 patients who participated in this study, 18 (45%) were female and 22 (55%) were male. The median age of the patients with mutation was 33 years of age, and the ones without mutation were 39.5. M1, M2and M4 FAB subgroups, with respectively 60, 37.5 and 35.7%, had the most occurrence of mutation. There was no significant relationship between mutation and the FAB subgroups (P=0.45). Favorable, intermediate and adverse cytogenetic risk groups had respectively 10, 37 and 66.7% mutations and 69.2% of the patients were in the normal karyotype group. Seventeen (42.5%) of the 40 patients achieved complete remission. 17.6% of them had mutations. There was no relationship between mutation and complete remission (P=0.085). Conclusion: There was no relationship between thepresence ofFLT3-ITD mutation and complete remission achievement following chemotherapy. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/225 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/225/218

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 21 23 Taleb Azarm Hematology- Oncology Department, Esfahan University of Medical Sciences, Tehran, Iran H Mohajer Internal Medicin Department, Esfahan University of Medical Siences, Tehran, Iran H Daghaghzadeh Gastrointrology Department, Esfahan University of Medical Sciences, Tehran, Iran Kamran Alimoghaddam Hematology- Oncology and Stem Cell Research Center, Tehran University of Medical Sciences,Tehran, Iran Mohammad Jahani Hematology- Oncology and Stem Cell Research Center, Tehran University of Medical Sciences,Tehran, Iran Ardeshir Ghavamzadeh Hematology- Oncology and Stem Cell Research Center, Tehran University of Medical Sciences,Tehran, Iran 2015 10 01 Introduction: A study was made conserning 142 patients who were suffering from gastrointestinal lymphoma, were seen at the department of Hematology and Oncology, Esfahan Medical School, during the period 1982-2002. Method: All patients had laparotomy, and biopsy of the site lesion. Histopathological subtypes were done in the International Working Formulation. Stage groupings were done applying the Crowther and Blackledge staging system. The post-laparotomy was made depending on the patient's general condition, the completeness of surgery and histological subtype. Results: There were 52 cases of gastrointestinal lymphoma in the stomach, 49 in the small intestine, 12 in the ileocecal region, 22 in the rectosigmoid and in seven of the cases, multiple sites in the gastrointestinal tract were involved. Conclusion: The overall survival rate was 47.8% at 5 years. Early stage disease and high-grade lymphoma have a better prognosis if treated adequately. Patients who had complete surgical removal of primary tumour (befor any metastasis) had a longer survival. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/226 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/226/520

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 25 33 Mehrdad Payandeh Hematology-Oncology Department, Faculty of Medical Science, Kermanshah University of Medical Science,Kermanshah, Iran Mehrnoush Aeinfar Hematology-Oncology Department, Faculty of Medical Science, Kermanshah University of Medical Science, Kermanshah, Iran Vahid Aeinfar Electronic Department, Faculty of Technology, Razi University, Kermanshah, Iran Computational Intelligence Research Center, Razi University, Kermanshah, Iran Mohsen Hayati Electronic Department, Faculty of Technology, Razi University, Kermanshah, Iran Computational Intelligence Research Center, Razi University, Kermanshah, Iran 2015 10 01 This paper represents a novel use of artificial neural networks in medical science. The proposed technique involves training a Multi Layer Perceptron (MLP) (a kind of artificial neural network) with a BP learning algorithm to recognize a pattern for the diagnosing and prediction of five blood disorders, through the results of blood tests from H1 machine. The blood test parameters and diagnosis of physician about the diseases of 450 patients from Taleghani Hospital in Kermanshah, Iran, are used in a supervised training method to update network parameters. This method was implemented to diagnose these disorder and cancer: Megaloblastic Anaemia, Thalassemia, Idiopathic thrombocytopenic pupura (ITP), Chronic myelogenous leukemia and Lymphoproliferative. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/227 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/227/220

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 34 37 Mozaffar Aznab Hematology-Oncology Department, Kermanshah University of Medical Science (KUMS) A Tabarroki Hematology-Oncology Department, Kermanshah University of Medical Science (KUMS) Gh Mohsseni Hematology-Oncology Department, Kermanshah University of Medical Science (KUMS) F Farshian Faculty of Medical Sciences, Kermanshah University of Medical Science (KUMS) K Kavyani Statistic Department, Kermanshah University of Medical Science (KUMS) 2015 10 01 Introduction: Colo-rectal cancer has 10% prevalence, among all of the cancer proportionally and also it is the third common cancer in the both sexes. Two recently introduced active drugs in the treatment of advanced colorectal cancer (ACC) are irinotecan and oxaliplatin. The combinations of oxaliplatin (OXA) or irinotecan (IRI) with 5FU-LV have been accepted as standard treatment for metastatic colorectal cancer. Patients and Methods: fifty four patients with colo-rectal cancer who came to the Oncology Clinic of Kermanshah University were assessed over a period of 4 years (2005-2008). All cases in stage III were treated by FOLFOX, unlike the patients in Stage IV treated with FOLFOX during 8 cycles fallowed by FOLFIRI in the same cycles (Sequential method). Results: the age average was less (49.1 years versus 55 years) than in other studies (6). A parallel analyzation of solid data, overall survival (OS), progression free survival (PFS) were 18 and 17.3 months, respectively. Conclusion: FOLFOX and FOLFIRI were administrated in 8 cycles each concomitantly (Sequential form) which provided considerable response with manageable complications. The result of the treatment in the study was correlated with other trials utilizing more modern procedures of medication like ‘Target therapies’ (OS; 18.4m for CT versus 19-20m for target therapies). https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/228 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/228/221

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 4 2009 12 01 38 42 Mohammadali Mashhadi Hematology- Oncology Department, Zahedan University of Medical Sciences, Zahedan, Iran K Nazem Gastroentrology Department, Zahedan University of Medical Scienceshaveisi Zadeh Medical Genetics Department, Faculty of Medicine, Shahid Beheshti University of Medical Sciences and Heaith Services, Tehran, Iran 2015 10 01 Myeloproliferative neoplasms (MPNs) such as polycythemia vera, essential thrombocythemia, primary myelofibrosis and chronic myeloid leukemia have too similar and accurate way to differentiate their is study of genetic disorders in these patients. Philadelphia chromosome is a sure way to definitively diagnose CML. Recently, JAK2V617F mutation introduced as a diagnostic marker for other Myeloproliferative neoplasms. Many studies show that the absence of the JAK2 mutation in chronic phase Philadelphia positive CML. In contrast with these reports, more recently, several cases with the coexistence of Philadelphia positive chromosome and JAK2V617F mutation in blood and bone marrow samples were reported. Here, we report a patient that have the Philadelphia chromosome disorder and JAK2V617F mutation in same time. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/278 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/278/271

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 5 2 2011 06 15 38 40 Nasim Valizadeh Hematology- Oncology Department, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran Negar Haghighi Internal Medicine Department, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran Negar Aghamohammadi Internal Medicine Department, Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran Mohammad Mohammadzadeh Vitreo- retinal Subspecialty ward, Ophthalmology Department, Shafa Hospital, Urmia University of Medical, Sciences, Urmia, Iran 2015 10 01 Central serous chorioretinopathy (CSCR) is an ocular disease which fluid is collected under retina and leads to retinal detachment and blurred vision. Risk factors for development of central serous chorioretinopathy are included: alcohol use, uncontrolled hypertension, pregnancy, type A personalityand different ways of corticosteroid use. We want to present central serous chorioretinopathy in a case of B-cell type Non-Hodgkin lymphoma (NHL)after taking 6th course of CHOP regimen which was presented with left- sided blurred vision. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/279 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/279/272

titter on Hela cells. Then, the CD34+ cells of cord blood can be pre-stimulated in a serum- free media supplemented with SCF, Flt3,TPO,IL6 in the presence and absence of 50 ng/ml MIP-1α. Transduction efficiency was assessed by a semi-quantitative PCR for the neomycin gene. Results: A PCR analysis of the neomycin gene in CD34+ cells revealed an improved transduction of cord blood cells in the presence of MIP-1α 65%, in comparison to its absence: 40.7%. Conclusion: the addition of MIP-1α to the cytokine cocktail improves the transduction efficiency of cord blood hematopoietic progenitor cells. Further studies are required to clarify its effect on the functional properties of CD34+ cells. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/212 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/212/205

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 3 2 2009 06 15 27 30 Shahrbanou Keyhanian Oncology- Hematology Department, School of Medicine, Islamic Azad University, Tonekabon, Mazandaran, Iran O Taziki Nephrology Department, Medical University of Mazandaran MM Saravi Radiology Department, Imam Sajjad Hospital, Ramsar, Mazandaran, Iran Z Fotokian School of Nursing and Midwifery, Ramsar, Babol Medical Sciences university, Mazandaran, Iran 2015