Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 195 199 EN Mohammad Vaezi Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Mahboubeh Vala Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Maryam Souri Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Ashraf Mousavi Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Ardeshir Ghavamzadeh Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran 2015 12 21 2016 05 12 Background: Social, occupational and emotional adjustment of Oncology nurses were assessed and compared with other nurses in this study. Subjects and Methods: One hundred nurses including Oncology nurses (n=50) and non-Oncology nurses (n=50) participated in cross-sectional study conducted in Shariati Hospital. Bell's Adjustment Inventory was used to measure social, emotional and occupational adjustment. Survey data were entered into SPSS statistical software, version 18 and the Kruskal-Wallis test was used for data analysis. Results: The study included nurses from Women’s Internal Medicine ward (14%), Men’s Internal Medicine ward (13%); Midwifery unit (17%), Operating room (15%) and Hematology-Oncology ward (41%). The mean age of the participants was 36.98 ± 8.28 years. In group of Hematology-Oncology nurses, the mean scores for occupational, social and emotional adjustment were 13.23 ± 1.99, 12.47 ± 1.79 and 18.19 ± 2.52, respectively. Data analysis showed that there is a statistically significant difference in the mean score of three areas of adjustment between Oncology nurses and their colleagues working in general wards (p-value=0.002, p-value<0.001, p-value<0.001 for occupational, social and emotional adjustment, respectively). Conclusion: The results of the study indicated that Oncology nurses had significantly lower social, occupational and emotional adjustment compared with nurses working in other wards. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/537 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/537/510

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 200 205 EN Alireza Abdollahi Associate Professor of Pathology, Department of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Mehrnoosh Etemadi Students of Scientific Research Centre, Tehran University of Medical Sciences, Tehran, Iran 2015 10 14 2015 11 21 Background: Triple-negative breast cancers (TNBC) are defined as breast cancers with lack of estrogen and progesterone receptors and no overexpression of human epidermal growth factor receptor 2 (HER2). This study was performed to determine the frequency and pathologic features of TNBC in Iranian patients. Subjects and Methods: This cross-sectional study was performed on patients with breast cancer who referred to Cancer Institute, affiliated to Tehran University of Medical Sciences, from April 2014 to April 2015. Data about the demographics, the status of gene receptors and the pathologic features were extracted from patients’ records. Results: Of 214 pathology samples of patients with malignant breast cancer, TNBCs account for 14% of cases. The mean age in N-TNBC group was 50 ± 12 years. Significant difference was seen between the age of two groups (p=0.03). No significant difference was observed regarding the number of involved lymph nodes between two groups (p=0.058). Presence of vascular and nerve invasion and involvement of surgical margins at the time of diagnosis were significantly more frequent in TNBC group comparing with N-TNBC. Grade III of histologic and nuclear grading was significantly more common in TNBC. Conclusion: TNBC group was significantly associated with higher grade, higher mitotic indices and higher rate of P53 positivity and higher level of Ki-67 at the time of diagnosis. High grade breast cancers are more seen in TNBC. The presence of aforementioned characteristics in a patient highlights the need for evaluating TNBC biomarkers to better predict prognosis and consider appropriate treatment. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/377 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/377/512

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 206 211 EN Babak Abdolkarimi Assistant Professor of Hematology-Oncology, Department of Pediatrics, Lorestan University of Medical Sciences, Khoramabad, Iran Soheila Zareifar Associate Professor of Hematology-Oncology, Department of Pediatrics, Amir Oncology Hospital, Shiraz University of Medical Sciences, Shiraz, Iran Majid Golestani Eraghi Fellowship in Intensive Care, Anesthesiologist, Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran. AND Fellowship in Intensive Care, Anesthesiologist, Lung Transplantation Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran Fazl Saleh fellowship of hematology oncology Department of pediatrics Shiraz Amir oncology Hospital, Shiraz University of Medical science 2015 11 17 2016 03 10 Background: Children suffering from cancer always require pain relief and reduce anxiety when undergoing painful procedures. The aim of this study is to compare the effect of pethedine and ketamine administration in cancer-diagnosed children undergoing bone marrow aspiration and biopsy procedures. Subjects and Methods: A randomized, double-blinded, crossover trial was carried out on 57 children undergoing painful procedures (bone marrow aspiration/biopsy). Patients were randomly assigned in a double-blinded fashion to receive either intravenous pethedine (1 mg/kg/dose) or ketamine (1 mg/kg/dose), respectively. The effectiveness of the drug was measured utilizing three parameters; perception of procedural pain with Wong-Baker Faces Pain Rating Scale and Richmond Agitation-Sedation Scale (RASS), hemodynamic changes and respiration and the frequency of vomiting nausea score. Results: Additionally, hemodynamic stability and pain control were significantly better in the patients receiving ketamine (p<0.05, at 0, 15, 30 min). Nausea and vomiting were more frequent in Group K than in Group M but there were no significant differences. No serious complications were observed. Conclusion: This study showed that intravenous ketamine generated a superior clinical effect in decreased pain. Ketamine may also be recommended as a reasonable option before oncology procedures in children suffering from cancer.   https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/510 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/510/513

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 212 216 EN Mohammad Ali Mashhadi Professor, Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Zahra Sepehri Assistant Professor of Internal Medicine, Department of Internal Medicine, Amir Al-Momenin Hospital, Zabol University of Medical Sciences, Zabol, Iran Ali Reza Bakhshipour Associate Professor, Department of Internal Medicine, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran Ali Zivari Assistant Professor, Department of Internal Medicine, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran Hossein Ali Danesh Assistant Professor of Surgical Medicine, Department of Surgical Medicine, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran Hasan Ali Metanat Assistant Professor, Department of Internal Medicine, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran Azra Karimkoshteh Assistant Professor, Department of Internal Medicine, Ali-Ebne Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran Seyed Mehdi Hashemi Professor, Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran Hossein Rahimi Assistant Professor of Internal Medicine, Department of Internal Medicine, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran Zohre Kiani Student Research Committee, Zabol University of Medical Sciences, Zabol, Iran. AND Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran 2015 10 20 2015 10 21 Background: Combination chemotherapy is accepted as a high efficacy treatment for gastric cancer, whereas choice of standard treatment is unclear. Multiple chemotherapeutic regimens have been used to achieve higher efficacy and lower toxicity. This study was designed to evaluate the treatment results of advanced gastric cancer with Capecitabine and Oxaliplatin regimen. Subjects and Methods: All cases with documented gastric adenocarcinoma and advanced disease were candidates for receiving Xelox regimen (Capecitabine – 750 mg/m2/twice daily/ 1-14 days and Oxaliplatin 125 mg/m2 in 1st day). Results: Twenty five cases with advanced gastric cancer entered in study while 24 cases continued treatment protocol and were evaluated. Mean age was 59.5 ± 12.1 years (range: 20-75), male and female cases were 66.7% and 33.3%, respectively. All cases received at least four cycles of Xelox regimen. Overall response rate was 74.99% with 29.16% complete response. Overall survival rate was 13 ± 0.53 months and DFS (disease-free survival) was 6 ± 1.09 months. Extremities neuropathy (62.5%), headache (45.8%) and muscle cramps (29.2%) were the most common complains. Haematological changes were rare and 16.7% of cases had mild cytopenia. Treatment related death was not observed. Conclusion: Xelox regimen is a safe and highly effective first line treatment for gastric cancer; however, considering it as first line therapy needs larger studies. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/479 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/479/514

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 217 223 EN Ahmed A. Al Ibraheemi Clinical Oncology Resident Doctor, Clinical Oncology and Radiation Therapy Department, Al-Bashir Hospital, Al-Ashrafiyah, Amman, Jordan Shaimaa Shamoun Oncology Nurse Specialist, Clinical Oncology and Radiation Therapy Department, Al-Bashir Hospital, Al-Ashrafiyah, Amman, Jordan 2015 10 13 2015 10 15 Background: Oral Mucositis (OM) remains the most common side effect of chemotherapy affects negatively on patients' quality of life. Subjects and Methods: Convenience samples of patients who received chemotherapy were followed from first or second cycle of chemotherapy until OM occurrence. We reviewed 75 female patients with breast cancer who received chemotherapy with mean age (47.2 SD ± 8.62861). We used WHO scale to assess the severity of OM. Demographic and other variables (age, number of cycle before appearance of signs of OM, WBC count, neutropenia count, creatinine and BMI) were filled in questionnaire. Results: 81.3% of reviewed patients were suffering from OM and (52.4%) of them were shown score 2 according to WHO classification, Taxane included chemotherapy protocol was the only significant variable that associated with OM occurrence (p=0.009). Conclusion: In this study; Taxane is the only risk factor that significantly associated with occurrence of OM. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/366 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/366/515

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 10 4 2016 10 06 224 231 EN Meysam Seyedifar Pharmaceutical Management and Economics Research Center, Tehran University of Medical Sciences, Tehran, Iran Farid Abedin Dorkoosh Department of Pharmacoeconomics and Pharmaceutical Administration, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Amir Ali Hamidieh Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Majid Naderi Department of Pediatrics Hematology-Oncology, Ali-Ebne Abitaleb Hospital Research Center for Children and Adolescent Health [RCCAH], Zahedan University of Medical Sciences, Zahedan, Iran Hossein Karami Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran Mehran Karimi Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Masoomeh Fadaiyrayeny Hematology-Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran Masoumeh Musavi 4Department of Pediatrics Hematology-Oncology, Ali-Ebne Abitaleb Hospital Research Center for Children and Adolescent Health [RCCAH], Zahedan University of Medical Sciences, Zahedan, Iran Sanaz Safaei MSc Student of Nursing, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran Mohammad Mahdi Ahmadian-Attari Department of Traditional Medicine, Medicinal Plants Research Center of Barij, Kashan, Iran Molouk Hadjibabaie Research Center for Rational Use of Drugs, Tehran University of Medical Sciences, Tehran, Iran. AND Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Abdol Majid Cheraghali Faculty of Pharmacy, University of Baqiyatallah Medical Sciences, Tehran, Iran Ali Akbari Sari Department of Health Management and Economics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran urnal> 29 34 EN R. Gupta Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India K. Rahman Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India M. Singh Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India S. Kumari Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India G. Yadav Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India S. Nityanand Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, India 2017 01 07 2018 01 02 Background:  Diagnosis of myelodysplastic syndromes (MDS) is challenging in the presence of morphological mimickers. Flow cytometric immunophenotyping (FCI) has been added to the diagnostic armamentarium, but its use in clinical practice is variable. Materials and Methods: Bone marrow aspirate samples from 54 patients with a clinical and/or morphological suspicion of MDS were subjected to FCI using a single-tube, 6-colour panel comprising of monoclonal antibodies against CD13, CD11b, CD16, CD34, CD45 and CD56. Analysis was centered on the abnormal maturation pattern of granulocytes, blast percentage (≥3%) and ratio of side scatter peak channel value (SSC-PCV) of granulocytes and lymphocytes. Each of these parameters was assigned a score of 1. Overall sensitivity and specificity of this panel was ascertained to differentiate cytopenia/s of MDS from non-MDS cases. Results: Forty MDS and 14 non-MDS cases were diagnosed based on morphology and cytogenetic results. Twenty control samples were also processed simultaneously for FCI to assign the cutoff for various flow cytometric parameters. A score ≥2 was defined as positive for MDS. Hypogranularity was present in 62.5% cases of MDS. The median SSC-PCV of granulocytes and lymphocytes was 6.16 in the MDS group, 7.9 in the non-MDS group and 8.90 in the control group (p <0.05). This cut-off value of 6.16 had a specificity of 92.5% based on the ROC curve analysis. Abnormal granulocyte maturation patterns for CD13/16, CD13/11b and CD11b/16 dot plots were observed in 95.3, 69.8 and 74.4% cases, respectively. The overall sensitivity and specificity of the panel was found to be 87.5% and 64.2%, respectively. Conclusion:  FCI is now an important tool for diagnostic workup in patients presenting with persistent cytopenia with or without morphological evidence of dyspoiesis. Inclusion of objective parameters like SSC-PCV would also reduce inter-lab variability in MDS diagnosis.   https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/728 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/728/595

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 12 1 2018 01 05 35 42 EN Behrooz Ghezelbash Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Azita Azarkeivan Pediatric Hematology Oncology, Iranian Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Thalassemia Clinic, Tehran, Iran Ali Akbar Pourfathollah Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Mohammad Reza Deyhim Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Biochemistry Department, Tehran, Iran Esmerdis Hajati Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Flow Cytometry Department, Tehran, Iran Alireza Goodarzi Laboratory Hematology and Blood Bank, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 2017 02 04 2018 01 02 Background: Some of the red cell storage lesions (RCSLs) take place during red blood cell (RBC) storage and may reduce the function of these cells dramatically, which mostly caused by residual leucocytes in blood components. This study was planned to observe the biochemical and hematological changes in pre-storage leukoreduced RBC (LR-RBC) compared with unfiltered RBC during in vitro storage.  Materials and Methods: Ten unit RBCs were collected, processed and stored according to Iranian standard operating procedure (SOP) of Iranian Blood Transfusion Organization (IBTO). Every unit was split into two equal parts, unfiltered RBC and LR-RBC. Samples were collected and tested on weeks of storage. Biochemical parameters such as lactate dehydrogenase (LDH), lactate concentration and glucose-6-phosphate dehydrogenase (G6PD) enzyme activity were measured by auto-analyzer. In addition, hematology analyzer was used to monitor the change of RBC indices such as (MCV), (MCH) and (MCHC). Results: In this study, both groups showed progressive increase of LDH and lactate levels, and also G6PD activity decreased during storage. Mean of LDH and lactate in unfiltered RBC was significantly increased compared with LR-RBC during all days of storage (p< 0.05). There was statically significant decrease in the G6PD enzyme activity between the two groups and weeks of storage (p< 0.05). However, the RBC indices remained within the expected levels in both groups. Conclusion: LR-RBC and RBC both exhibited RCSL during storage, but LR-RBC is effective in reducing Red cell storage lesion (RCSL) and also improves the quality of stored red blood cells. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/744 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/744/596

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 12 1 2018 01 05 43 48 EN Majid Naderi Genetic Researcher Center in Non-Communicable Disease, Zahedan University of Medical Sciences, Zahedan, Iran Manijeh Habibpour Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Shaban Alizadeh Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Zahra Kashani Khatib Hematology Department, High Institute for Research and Education in Transfusion Medicine, Iranian Blood Transfusion Organization, Tehran, Iran Akbar Dorgalaleh Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran Mohammed Awal Issah Department of Hematology, International Campus, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran Fatemeh Naadali Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran 2016 10 16 2018 01 02 Background: Glanzmann Thrombasthenia (GT) is a rare autosomal disease. HPA (Human Platelet Alloantigen) is a surface polymorphic alloantigen of platelets. This study was intended to investigate and compare the polymorphism of HPA-1 and HPA-5 genes in two groups of GT patients, with and without resistance to platelet and recombinant factor VII therapy. Materials and Methods: This case control study was performed on GT patients (n=16) with resistance to platelet therapy and recombinant factor VII and control group of GT patients (n=16) without resistance to platelet therapy and recombinant factor VII. The consent form was completed by each patient. Gene polymorphisms of HPA-1 and HPA-5 were investigated using SSP-PCR, and the obtained data were analyzed using statistical software SPSS16.0. Results: The results indicated no significant relationship between the studied genes and their resistance to platelet therapy and recombinant factor VII. The frequencies of HPA-1 genotype a/a were 98% and 94% in patient and control groups, respectively. The frequency of allele b was found to be less than allele a. The value of this allele was 4% in patient group and 1% in control group. In addition, the HPA-5a/a (98%) was the most frequent alloantigen?? (check it) in both groups. Seven percent (7%) of the patients had the HPA-5a/b genotype, and the HPA-5b/b was found to be absent in these individuals. Conclusion: According to the results obtained, it could be concluded that these genes play no role in resistance to platelet therapy.   https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/685 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/685/597

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 12 1 2018 01 05 49 56 EN