Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 4 8 Farzad Mollahoseini Foomani Blood Transfusion Organization Research Center, Iranian Blood Transfusion Organization, Mashhad, Iran Mahammad Hadi Sadeghain Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Saeede Bagheri Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Zahra Badiee Pediatrics Department, Doctor Sheikh Hospital, Mashhad University of Medical Sciences, Mashhad, Iran Reihane Bazargani Blood Transfusion Organization Research Center, Iranian Blood Transfusion Organization, Mashhad, Iran Zahra Aryanpour Blood Transfusion Organization Research Center, Iranian Blood Transfusion Organization, Mashhad, Iran Saeid Hallajian Blood Transfusion Organization Research Center, Iranian Blood Transfusion Organization, Mashhad, Iran Seyyede Fatemeh Shams Blood Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 2020 10 31 2021 05 01 Background:  Thalassemia is an inherited disease with anemia and hemolysis. Blood transfusion is a routine treatment for thalassemia patients; alloimmunization is one of the complications of blood transfusion, which is very serious for these patients, especially girls and young women.    Materials and Methods:  Four hundred forty-six thalassemia patients were examined in this cross-sectional study. Demographic information of patients was extracted and recorded. The phenotype of ABO, Rh, and Kell antigens (tube method) with antisera from IMMUNDIANOSTICA Company (Germany) and the frequency of alloantibodies were determined. Results: 55.8% of the studied individuals were male and 44.2% were female. The mean age of the studied patients was 19.94±10.63.  The alloantibodies were detected in 7.5% of pack cell receivers. The most prevalent phenotype of the ABO system was the O blood group (37.4%), and the most abundant antigen of the Rh group was ‘e’, which was found in 99.8% of the studied population. The most frequently detected alloantibody was Anti K (38.2%); concerning kell phenotype, (K_k+) and (K+k+) were found in 99.3% and 0.7% of patients, respectively. The frequency of Anti-D, Anti-C, Anti-c, and Anti-E was 23.5%, 14.7%, 2.9%, and 14.7%, respectively. Conclusion: According to the results of this paper, finding the compatible pack cells in terms of Kell and Rh systems antigens in addition to the ABO blood group is recommended to decrease the rate of alloantibodies in thalassemia patients. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1453 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1453/957

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 1 3 Ali Akbar Pourfathollah Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Karim Shamsasenjan Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Mahmoud Hadipour Dehshal Mehregan Pharmacy, Tehran, Iran 2022 08 22 2023 01 07 No Abstracts  No Abstracts  No Abstracts  https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1899 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1899/956

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 56 62 Rachel Hicks Brookdale University Hospital Medical Center Mohammed Alsabri Brookdale University Hospital Medical Center Mario Peichev Brookdale University Hospital Medical Center Viswanathan Kusum Brookdale University Hospital Medical Center 2020 11 02 2021 05 10 Periorbital swelling is a clinical presentation with a broad differential and potentially deleterious consequences. Causes range from benign, including allergic reaction, to vision- and life-threatening, including orbital cellulitis and orbital infarction. The recent climate of SARS-CoV-2 has further complicated this differential, as the virus poses broad clinical presentations with new manifestations reported frequently. Rapid identification of the underlying etiology is crucial, as treatment approaches diverge greatly. Here, we report the case of an African American adolescent male with a history of homozygous sickle cell anemia presenting to an inner city hospital with bilateral periorbital swelling amid the coronavirus pandemic. Differentials including orbital cellulitis, COVID-MIS-C, orbital inflammatory syndrome, Hoagland sign, and orbital infarction secondary to sickle cell crisis are contrasted. We contrast our case with 12 case reports of orbital infarction in the setting of sickle cell crisis within the past 10 years, highlighting how these presentations, along with commonly reported findings of orbital infarction, compare with our patient. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1462 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1462/963

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 9 17 EN Shahram Shojaei Bistoon Hospital, Kermanshah, Iran Mozaffar Aznab Department of Internal Medicine, Talaghani Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran Amirmasoud Rahimi Kermanshah University of Medical Sciences, Kermanshah, Iran Seyed Mojtaba Ahmadi Department of Clinical Psychology School of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran Kumars Eslampia Department of Surgery, Bistoon Hospital, Kermanshah, Iran Arash Golpazir Department of Surgery, Cancer Surgery, Kermanshah University of Medical Sciences, Kermanshah, Iran Masoud Iravani Masoud Gastroenterology and Liver Clinic, Tehran, Iran 2020 09 23 2021 05 09 Background: This study aimed to evaluate the significance of tumor lymphocyte infiltration (TIL) and the number of CD8+ T cells in breast cancer and their relationship with the other clinicopathological factors and overall survival (OS) was investigated. Materials and Methods: The studied samples were breast cancer patients (2005-2017) referring to the medical oncology departments for treatment. Pathologic samples of breast cancer patients were evaluated in terms of TIL and positive immunohistochemical staining for CD8 cytotoxic cells. Results: 299 patients were entered into the study, 3 male and 296 female. Their mean follow-up period was 61 months. Statistical findings indicated that lymph involvement is more accompanied by low TIL within the tumor (0.011). Correlations were observed between the estrogen, progesterone receptors, P53 state, and TIL; which were significant by P-value<0.049, P-value=0.024, P-value =0.002, respectively. With any Ki67 value, the number of patients with less than 30% TIL was more considerable than the two other groups with lymphocyte cut-off of 30-50% and more than 50%. Comparison of the OS of patients with positive and negative CD8 cytotoxic lymphocytes in 45 patients with lymphocyte infiltration of equal or more than 40% showed that the OS results were in favor of patients with CD8+ cytotoxic lymphocyte (0.022). Out of 299 patients, 17 died. Conclusion: Our findings showed that in cases of CD8+ cytotoxic lymphocytes in tumors, the OS of the patients will be enhanced which can act as an independent.   https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1432 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1432/958

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 18 27 Seyedeh Sahar Mortazavi Farsani Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Dina Sadeghizadeh Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Sadegh Babashah Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Fariba Rad Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran Majid Sadeghizadeh 1) Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran 2) Alborz Nanomed Technology Company, Tehran, Iran 2020 12 31 2021 05 31 Background: Megakaryopoiesis is characterized by progressive polyploidization and the expression of megakaryocytic markers. Numerous transcription factors and physiological signaling pathways regulate this phenomenon. Megakaryocyte differentiation induction in the K562 cell line and hematopoietic stem cells via nanocurcumin drug has been identified in our previous study. K562 cells are typical Chronic Myelogenous Leukemia (CML) cells that are resistant to apoptosis and express the bcr-abl fusion gene. These cells have the potential to differentiate into erythrocytes and megakaryocytes. Curcumin is well known as a component with strong potential to alter NFκB activity in various cells. NFκB pathway regulates various genes such as apoptotic and immune response genes. The aim of the current study is to evaluate the possible role of nanocurcumin in NFκB pathway regulation during the megakaryopoiesis process in the K562 cell line. Materials and Methods: Megakaryocyte markers expression and phenotype alteration of nanocurcumin-treated K562 cells have been detected by flow cytometry and microscopy imaging. The nuclear level of the RelA (p65) subunit of NFκB was determined by western blot test in K562 cells during megakaryopoiesis induction via nanocurcumin treatment at different times. The expression of NFκB target genes including c-MYC, BAX, and NQO1 was also analyzed in nanocurcumin-treated K562 cells by quantitative RT-PCR assay at different times. Results: It was demonstrated that nanocurcumin leads to an increase in NFκB activity transiently during megakaryocyte differentiation, which is followed by a change in the expression of c-MYC, BAX, and NQO1 target genes. Conclusion: The NFκB pathway can be considered a new pathway for inducing megakaryocyte differentiation by nanocurcumin for the purposes of in vitro and in vivo megakaryopoiesis experiments. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1514 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1514/959

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 28 38 Carlos Atencia-Flórez Department of Internal Medicine, University of Antioquia School of Medicine, Medellín, Colombia Catalina Quintero-Valencia Laboratorio Integrado de Medicina Especializada – LIME, Medellin (Antioquia), Colombia María Mondragón-Arismendy Laboratorio Integrado de Medicina Especializada – LIME, Medellin (Antioquia), Colombia Andrés Cardona-Arias University of Antioquia School of Medicine, the SURA Healthcare Insurance Company, Medellin (Antioquia), Colombia Carlos Regino-Agamez Department of Internal Medicine, University of Antioquia School of Medicine, Medellín, Colombia Julián Vélez-Urrego San Vicente Foundation University Hospital, Medellin (Antioquia), Colombia 2021 01 08 2021 05 05 Background:  Multiple myeloma is the second most common hematologic malignancy after lymphomas. Few studies have characterized significant and full variables at the time of diagnosis of multiple myeloma in Colombia, and there is no data evaluating patients for follow-up. Materials and Methods:  A retrospective cohort study is presented, describing the clinical, laboratory, cytometric, and cytogenetic characteristics of patients with a de novo diagnosis of multiple myeloma evaluated in a reference hematology laboratory attached to a highly complex hospital in Medellín, Colombia.  We follow them until death as a main outcome. Results:  A total of 170 patients with a de novo diagnosis of multiple myeloma were collected from a database of 421 patients with different monoclonal gammopathies. Mainly, it was found that 50.8% of the patients were men; the median age was 62 years; 65.4% had secretion of the IgG kappa; half of the patients presented International Staging System (ISS) Stage III. The β2 macroglobulin >4 mg/L and creatinine >2 mg/dl were the main variables significantly associated with survival (Hazard Ratio (HR) 2.4 and 2, respectively). Eighty-five percent of patients presented with bone lytic lesion involvement and less than 3% with extramedullary involvement. Conventional Banding Karyotype (CBK) genetic risk assessment yield was poor, compared with although scarce data regarding Cytogenetic risk assessment based on Fluorescence in-situ Hybridization (FISH). Conclusion:  The clinical profile of the patients with a de novo diagnosis of multiple myeloma in our cohort is similar to that described in international studies. The diagnosis of multiple myeloma was documented at younger ages, with more advanced stages, anemia, and a high percentage of bone disease. ISS provides an excellent tool for prognosis purposes. Cytogenetic risk assessment based on FISH should be done for all MM patients from therapeutic implications. We need standardized protocols for bone marrow sample manipulation and processing in order to guarantee good correlation for plasma cells count methods. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1522 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1522/960

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 17 1 2023 01 16 39 kin lymphoma (NHL) and classical Hodgkin lymphoma (HL), and is curative in ~40% to 45% of patients. Over a decade many efforts were made to find helpful parameters to predict an optimal time for initiating an efficient peripheral blood stem cell collection so that adequate stem cells are collected.  It has been well accepted that CD34+ cell count in peripheral blood before leukapheresis is the best parameter to predict CD34 cell yield. However, white blood cell count, mononuclear cell count, and other easily obtained parameters are still used to guide the clinical practice of peripheral blood stem cell mobilization and collection.  Materials and Methods: In the present study, we analyzed the correlation between peripheral blood MNC and Apheresis CD34 levels and also between peripheral blood CD34 by flow cytometry and apheresis CD34 levels. Results: We found that there was a statistically insignificant weak correlation between peripheral MNC and apheresis CD34. There was a statistically significant strong correlation between peripheral CD34 and apheresis CD34. Conclusion: The results show that peripheral blood MNC was analogous indicating that no reliable prediction can be done for CD34 cells collected in apheresis while peripheral CD34 by flow cytometry is the strongest predictor for initiating stem cell collection. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1261 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1261/882

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 15 3 2021 07 14 178 191 EN Mohamed Bahr Department of Surgery, Faculty of Veterinary Medicine, Cairo University, Egypt Mohamed Amer Department of Surgery, Faculty of Veterinary Medicine, Cairo University, Egypt Khaled Abo-EL-Sooud Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Egypt Ahmed Abdallah Department of Pathology, Animal Health Research Institute, Cairo, Egypt Gehan Shehab Department of Pathology, Animal Health Research Institute, Cairo, Egypt Omar El-Tookhy Department of Surgery, Faculty of Veterinary Medicine, Cairo University, Egypt 2020 02 28 2020 07 21 Background: Fresh stem cell exosomes are usually obtained and reused in the same individual. It cannot be kept viable for a long period of time regardless of the lengthy preparation time. Freezing is typically used to preserve the viability of perishable materials and increase their lifetime. Regrettably, normal freezing of biomaterials leads to cell damage. Therefore, a cryoprotectant can save the cells from the conventional cryodamage. Sodium carboxymethylcellulose (NA-CMC) is a powdery substance that is used to manufacture bio-safe hydrofilm gels because of its high viscosity, cytocompatibility, and non-allergenic nature. Materials and Methods: Sterile CMC hydrogel was prepared, part of which was loaded with exosomal solution derived from MSCs. The gel was kept at −20°C for preservation. Two bilateral full-thickness circular skin wounds of 2-cm diameter were created on the back of experimental dogs. The wounds were at least 2.5 cm apart. Treatment started 24 hours after wound creation. Group I received CMC gel solely, whereas group II received frozen CMC exosomal gel. The gel was applied 4 times, a single application per day with 1-day interval. Results: Clinically, the frozen exosomal gel significantly promoted wound healing with no scaring. Histologically, enhanced dermal fibroblasts and organized collagen deposition were seen in the treated group. Conclusion: CMC proved to be an efficient cryoprotectant and a suitable vehicle for exosomes. Deep freezing was proven to conserve the viability, extended the preservation, and facilitated the usage of exosomal gel. This technique of preserved cell-free therapy is inexpensive, time-saving, and proficient and seems suitable for treating cutaneous wounds. https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1285 https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/download/1285/883

Tehran University of Medical Sciences International Journal of Hematology-Oncology and Stem Cell Research 2008-2207 15 3 2021 07 14 192 198 EN Sanaz Asiyabi Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Seyed Mahdi Marashi Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Rohollah Vahabpour Department of Medical Lab Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran Ahmad Nejati Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Alireza Azizi-Saraji Iranian Comprehensive Hemophilia Care Center, Tehran, Iran Aliyeh Sada