International Journal of Hematology-Oncology and Stem Cell Research 2017. 11(3):185-191.

Expression Analysis of Long Non-coding PCAT-1
Shaghayegh Sarrafzadeh, Lobat Geranpayeh, Soudeh Ghafouri-fard

Abstract


Background: The prostate cancer-associated non-coding RNA transcript 1 (PCAT-1) is a newly identified long non- coding RNA whose participation in tumorigenesis of a variety of cancers has been observed. In the present study, we aimed at analysis of its expression in breast cancer patients.

Subjects and Methods: The expression of PCAT-1 was assessed using real-time reverse transcription polymerase chain reaction in tumor samples obtained from 47newly diagnosed breast cancer patients as well as their corresponding adjacent non-cancerous tissues (ANCTs).

Results: We detected significant over-expression of PCAT-1 in 12/47 (25.5%) of tumoral tissues compared with their corresponding ANCTs. However, no significant association has been found between the levels of PCAT-1 transcripts and patients’ clinical data such as tumor size, stage, grade, estrogen and progesterone receptors or Her2/neu status.

Conclusion: PCAT-1 is possibly involved in the pathogenesis of fraction of breast cancers. Future studies are needed to evaluate its precise function in breast cancer.


Keywords


Breast cancer, lncRNA, PCAT1

Full Text:

PDF

References


Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015;65(1):5-29.

Waldemarson S, Kurbasic E, Krogh M, et al. Proteomic analysis of breast tumors confirms the mRNA intrinsic molecular subtypes using different classifiers: a large-scale analysis of fresh frozen tissue samples. Breast Cancer Res. 2016;18(1):69.

Dianatpour M, Mehdipour P, Nayernia K, et al. Expression of Testis Specific Genes TSGA10, TEX101 and ODF3 in Breast Cancer. Iranian Red Crescent medical journal. 2012;14(11):722-6.

Kazemi-Oula G, Ghafouri-Fard S, Mobasheri MB, et al. Upregulation of RHOXF2 and ODF4 Expression in Breast Cancer Tissues. Cell journal. 2015 Fall;17(3):471-7.

Derrien T, Johnson R, Bussotti G, et al. The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression. Genome Res. 2012;22(9):1775-89.

Nikpayam E, Tasharrofi B, Sarrafzadeh S, et al. The Role of Long Non-Coding RNAs in Ovarian Cancer. Iran Biomed J. 2017;21(1):3-15.

Soudyab M, Iranpour M, Ghafouri-Fard S. The Role of Long Non-Coding RNAs in Breast Cancer. Archives of Iranian medicine. 2016;19(7):508-17.

Iranpour M, Soudyab M, Geranpayeh L, et al. Expression analysis of four long noncoding RNAs in breast cancer. Tumour Biol. 2016;37(3):2933-40.

Tasharrofi B, Soudyab M, Nikpayam E, et al. Comparative expression analysis of hypoxia-inducible factor-alpha and its natural occurring antisense in breast cancer tissues and adjacent noncancerous tissues. Cell Biochem Function. 2016;34(8):572-578.

Guo HY, Ahmed M, Zhang F, et al. Modulation of long noncoding RNAs by risk SNPs underlying genetic predispositions to prostate cancer. Nat Genet. 2016;48(10):1142-50.

Prensner JR, Iyer MK, Balbin OA, et al. Transcriptome sequencing identifies PCAT-1, a novel lincRNA implicated in prostate cancer progression. Nat Biotechnol. 2011;29(8):742.

Proverbs-Singh T, Feldman JL, Morris MJ, et al. Targeting the androgen receptor in prostate and breast cancer: several new agents in development. Endocr-Relat Cancer. 2015;22(3):R87-R106.

Chia K, O'Brien M, Brown M, et al. Targeting the Androgen Receptor in Breast Cancer. Curr Oncol Rep. 2015;17(2).

Prensner JR, Chen W, Han SM, et al. The Long Non-Coding RNA PCAT-1 Promotes Prostate Cancer Cell Proliferation through cMyc. Neoplasia. 2014;16(11):900-8.

Ye J, Coulouris G, Zaretskaya I, et al. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC bioinformatics. 2012;13:134.

Ge X, Chen Y, Liao X, et al. Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer. Med Ooncol. 2013;30(2):588.

Yan TH, Yang H, Jiang JH, et al. Prognostic significance of long non-coding RNA PCAT-1 expression in human hepatocellular carcinoma. Int J Clin Exp Pathol. 2015;8(4):4126-31.

Zhao B, Hou X, Zhan H. Long non-coding RNA PCAT-1 over-expression promotes proliferation and metastasis in non-small cell lung cancer cells. Int J Clin Exp Pathol. 2015;8(10):18482-7.


Refbacks

  • There are currently no refbacks.


Creative Commons Attribution-NonCommercial 3.0

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.