Blood Group Discrepancies at a Regional Blood Center

  • Hayedeh Javadzadeh Shahshahani Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  • Azam Hayati Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Yazd, Iran
Blood group discrepancy; Blood donors; ABO blood typing


Background: Blood group testing is an important part of supplying safe blood components in blood transfusion centers. Blood group discrepancy develops when reactions in forward grouping do not correspond with reverse grouping or if the preceding and recent results do not match. This study aimed to evaluate ABO blood group discrepancies among blood donors of Yazd, Iran.

Materials and Methods: In this cross-sectional study, data of blood donors were obtained from the integrated database of Yazd Blood Transfusion Center during a period of eight years (2010 – 2017). Tube testing was used for determining the ABO blood groups. A serological workup was performed for diagnosis and determination of the discrepancy. Confirmation of the results was accomplished by the reference laboratory of immunohematology.

Results: Blood group discrepancies were detected in 130 (0.04%) out of 322,222 donations. Technical/Clerical errors leading to ABO discrepancy were noticed in 12 (9.3%) cases. The most frequent cause of ABO discrepancies in forward grouping was subgroups of A Antigen (44.6%) and in reverse grouping was cold autoantibody (23.9%). There were 11 (8.4%) cases with alloantibodies. Two blood donors with rare Bombay phenotype and p blood group were also identified.

Conclusion: For minimizing Technical/Clerical errors, accurate blood donor or sample identification programs should be implemented. All cases of blood group discrepancies should be carefully investigated, and blood donors should be informed appropriately.


1. Cooling L. ABO, H, and Lewis Blood Groups and Structurally Related Antigens. In: Fung MK, Grossman BJ, Hillyer CD, et al, (eds). Technical Manual. 18th edn, Bethesda, Maryland: American Association of Blood Banks; 2014, pp 297-313.
2. Chiaroni J, Legrand D, Dettori I, et al. Analysis of ABO discrepancies occurring in 35 French hospitals. Transfusion. 2004; 44(6): 860–4.
3. Sharma T, Garg N, Singh B. ABO blood group discrepancies among blood donors in Regional Blood Transfusion Centre GTB Hospital, Delhi, India. Transfus Apher Sci. 2014; 50(1):75-80.
4. Kaur G, Kaur P, Basu S, et al. Blood group discrepancies at a tertiary care centre – analysis and resolution. Int J Lab Hematol. 2014; 36(4):481-7
5. Makroo RN, Kakkar B, Agrawal S, et al. Retrospective analysis of forward and reverse ABO typing discrepancies among patients and blood donors in a tertiary care hospital. Transfus Med. 2019; 29(2):103-109.
6. Shahverdi E, Moghaddam M, Gorzin F. Maternal red blood cell alloantibodies identified in blood samples obtained from Iranian pregnant women: the first population study in Iran. Transfusion. 2017; 57(1):97-101.
7. Thakral B, Saluja K, Bajpai M, et al. Importance of weak ABO subgroups. Lab Med. 2005; 36(1): 32–4.
8. Chaurasia R, Rout D, Dogra K, et al. Discrepancy in Blood Grouping: Subgroups of B—Challenges and Dilemma. Indian J Hematol Blood Transfus. 2017; 33(4):628-629.
9. Klein HG, Anstee DJ. Mollison's Blood transfusion in Clinical Medicine. 11th ed. Oxford, UK: Blackwell; 2005, pp 209-252
10. Khalid S, Dantes R, Varghese S, et al. Naturally occurring anti M complicating ABO grouping. Indian J Pathol Microbiol. 2011; 54(1):170–2.
11. Benidt GR, Jaben EA, Winters JL, et al. Identification of anti-PP1P(k) in a blood donor and her family: a case report following her pregnancy and review. Transfus Apher Sci. 2010; 43(3):369-74.
12. Shahshahani HJ, Vahidfar MR, Khodaie SA. Transfusion reaction in a case with the rare Bombay blood group. Asian J Transfus Sci. 2013; 7(1): 86–87.
13. Mohd Azri MS, Kunasegaran K, Azrina A, et al. Successful Pregnancy Outcome in Malaysian Woman with Rare p Phenotype and Anti-PP1Pk Antibody. Indian J Hematol Blood Transfus. 2014; 30(Suppl 1): 405–408.
How to Cite
Javadzadeh Shahshahani H, Hayati A. Blood Group Discrepancies at a Regional Blood Center. Int J Hematol Oncol Stem Cell Res. 14(1):38-44.
Original Article(s)