The Antihelminthic Drug, Mebendazole, Induces Apoptosis in Adult T-cell Leukemia/Lymphoma Cancer Cells: In-vitro Trial

  • Amirhosein Maali 1) Infection Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran 2) Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran 3) Student Research Committee, Babol University of Medical Sciences, Babol, Iran
  • Elaheh Ferdosi-Shahandashti Mail 1) Infection Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran 2) Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran 3) Department of Medical Biotechnology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
  • Farzin Sadeghi Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
  • Ehsan Aali Department of Pharmacology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
Keywords:
Adult T-cell leukemia/lymphoma, Mebendazole, Drug switching, Apoptosis

Abstract

Background: Adult T-cell leukemia/lymphoma (ATLL) is a poor prognostic Hematopoietic malignancy with various therapeutic challenges, which had been classified as non-Hodgkin lymphoma.  The Drug switching, as a novel, innovative and promising approach, is an opportunity to overcoming on therapeutic challenges of hard-treating disease, e.g. ATLL. Our aim is evaluating the antiproliferative and apoptotic effect of Mebendazole (MBZ) on ATLL cancer cells in in-vitro conditions.

Materials and Methods: We used Jurkat cell-line as ATLL cancer cells. After treatment of MBZ in different concentrations on jurkat cells, the cell viabilities were determined by MTT assay. After IC50 value determination, the 24-, 48- and 72-h treatments had been performed in IC50 concentration and control to evaluating the quantitative apoptosis rate by Annexin/PI Flowcytometry and qualitative apoptosis by DAPI Nuclear staining. Also, Glucose spectrophotometry were performed to evaluate the reduced amount of glucose uptake through MBZ treatment.

Results: MBZ inhibits proliferation of jurkat cells and IC50 value had been estimated 10 μM (P< 0.01). According to the flowcytometric results, increasing in drug concentration is associated with decrease cell viability and the percentage of full-apoptosis. However, it inversely correlates with percentage of early-apoptosis rate. Also, the microscopic captures of DAPI Nuclear staining confirms the flowcytometry results in qualitative manner. In addition, it was found that inhibition of glucose uptake was inversely correlated with increased MBZ concentration (P< 0.05).

Conclusion: MBZ potentially inhibits the proliferation of ATLL cancer cells in in-vitro condition. MBZ inhibits the growth of Jurkat cells by inducing apoptosis. Also, we suggest that indirectly inhibition of Glucose transporting occurs by MBZ, which could induce apoptosis in cancer cells.

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Published
2020-10-07
How to Cite
1.
Maali A, Ferdosi-Shahandashti E, Sadeghi F, Aali E. The Antihelminthic Drug, Mebendazole, Induces Apoptosis in Adult T-cell Leukemia/Lymphoma Cancer Cells: In-vitro Trial. Int J Hematol Oncol Stem Cell Res. 14(4):266-273.
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Original Article(s)