The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells
Background: Irradiation leads to increased storage lesions that may have harmful effects if transfused. Various storage lesions research has been carried out, and only very few articles are available on the impact of gamma irradiation on RBC storage lesions. Since there has been no study about finding the best time for irradiation, we decided to investigate the effect of irradiation on Red blood cells at different storage times after blood collection
Materials and Methods: A total of 40 units of red blood cells divided into two groups: irradiated and non-irradiated. Irradiated RBCs were divided into three groups, each containing ten units. The remaining ten units were considered as non-irradiated controls. Sampling from these irradiated and non-irradiated blood units was performed weekly to evaluate biochemical parameters and free plasma hemoglobin/Hemolysis index levels.
Results: A significant increase in the mean values of plasma potassium, plasma Hb/Hemolysis index, and LDH, as well as a significant reduction in the mean value of 2,3 DPG and plasma sodium, were observed in both groups. Although the reduction of 2,3 DPG is extremely remarkable, it is compensated 24-48 hours after transfusion. Hence, the clinical result of 2,3-DPG-depleted RBC transfusion is known to be negligible. The irradiation group alteration was more notable than the non-irradiated one and the changes in the parameters were most significant in the group that was stored for a longer period after irradiation.
Conclusion: The investigation on the impact of gamma irradiation on RBCs makes it possible to suggest a storage time up to 28 days after irradiation and the best time for irradiation after blood collection is up to 14 days. It is pointed out that the blood unit should be transfused as soon as possible after the irradiation
2. Patel KK, Patel AK, Ranjan RR, et al. Transfusion associated graft versus host disease following whole blood transfusion from an unrelated donor in an immunocompetent patient. Indian J Hematol Blood Transfus. 2010; 26(3): 92–95.
3. Del Lama LS, de Góes EG, Petchevist PCD, et al. Prevention of transfusion-associated graft-versus-host disease by irradiation: technical aspect of a new ferrous sulphate dosimetric system. PloS one. 2013;8(6):e65334.
4. Agarwal P, Ray V, Choudhury N, et al. Effect of pre-storage gamma irradiation on red blood cells. Indian J Med Res . 2005;122(5):385-7.
5. Treleaven J, Gennery A, Marsh J, et al. Guidelines on the use of irradiated blood components prepared by the British Committee for Standards in Haematology blood transfusion task force. Br J Haematol . 2011;152(1):35-51.
6. European Directorate for the Quality of Medicines and Health Care. Guide to the preparation, use and quality assurance of blood components. 20th edn. Strasbourg, France: Council of Europe Publishing, 2020, pp 188-189.
7. D’Alessandro A, D’Amici GM, Vaglio S, et al. Time-course investigation of SAGM-stored leukocyte-filtered red bood cell concentrates: from metabolism to proteomics. Haematologica. 2012;97(1):107-15.
8. Adams F, Bellairs GR, Bird AR, et al. Metabolic effects occurring in irradiated and non-irradiated red blood cellular components for clinical transfusion practice: An in vitro comparison. Afr J Lab Med . 2018;7(1):606.
9. Reverberi R, Govoni M, Verenini M. Deformability and viability of irradiated red cells. Ann Ist Super Sanita. 2007;43(2):176-85.
10. Chaudhary R, Katharia R. Oxidative injury as contributory factor for red cells storage lesion during twenty eight days of storage. Blood Transfus. 2012;10(1):59-62.
11. Sawant R, Jathar S, Rajadhyaksha S, et al. Red cell hemolysis during processing and storage. Asian J Transfus Sci . 2007;1(2):47-51.
|Issue||Vol 15 No 1 (2021)|
|Red blood cells, Gamma irradiation, Storage lesions, Transfusion-associated graft versus host disease (TA-GVHD)|
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|This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.|