In Search of Mesenchymal Stem Cells: Bone Marrow, Cord Blood, or Peripheral Blood
,
Abstract
Objective: Mesenchymal stem cells (MSC) are capable of self-renewal and differentiation into various connective tissue lineages. Therefore, they have attracted a lot of attention from investigators in the context of stem cell therapies. In our study, we have evaluated the frequency, phenotype and differen¬tiation potential of MSC in bone marrow (BM) cord blood (CB) and mobilized peripheral blood (mPB).
methods: Sixteen CB, 11 BM and 19 mPB were obtained from normal donors. Mononuclear cells sus¬pended in culture medium and seeded in culture flasks. Flasks were incubated in a CO2 incubator with a change of culture medium every 4 days and passaged when fibroblast like cells reached confluence. For every other passage, MSC were examined for CD13, CD44,CD34 by flow cytometry and induced to differentiate into adipocytes and osteocytes.
Results: All BM samples produced MSC that survived multiple passages in mesenchymal culture me¬dium over 4 months. CB and mPB samples produced a non-confluent adherent layer of heterogeneous cells, and did not proliferate beyond the first passage. Immunophenotype of BM-derived MSC in every other passage were CD34-, CD13+ and CD44+, the adipogenic and osteogenic differentiation were con¬firmed by Oil-red O and Von Kossas staining, respectively. The mentioned evaluation for mPB and CB were not attempted because these were not confluent even in the first passage.
Conclusion: In our study, only human BM cells produced MSC. These cells are positive for MSC sur¬face proteins and differentiate into MSC lineages.
- Owen M. Lineage of osteogenic cells and their rela- tionship to the stromal system. In Bone and mineral re- search 3 (ed. W.A. Peck) 1985; pp 1-25 Elsevier, Am- sterdam. The Netherlands.
- Caplan A.I. Mesenchymal stem cells. J. Orthop. Res. 1991; 9: 641.
- Aubin J.E., Bellows C.G., Turksen K., et al. Analysis of the osteoblast lineage and regulation of differentiation. In Chemistry and biology of mineralized tissues (eds. H. Slavkin and P. Price). 1980; pp 267. Elsevier, Amester- dam, The Netherlands.
- Poliard A., Nifuji A., Lamblin D., et al. Controlled conversion of an immortalized mesodermal progenitor cell towards the osteogenic, chondrogenic, or adipogenic pathways. J. Cell Biol. 1995; 130: 1461.
- Young H.E., Mancini M.L., Wright R.P., et al. Mesen- chymal stem cells resides within connective tissues of many organs. Dev. Dyn.1995; 202: 137.
- Jiang Y, Jahagirdar BN, Reinhardt RI, et al. Pluripo- tency of mesenchymal stem cells derived from adult marrow. Nature 2002;418:41.
- Jaiswal N., Haynesworth S.E., Caplan A.I., et al. Os- teogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. Journal of Cel- lular Biochemistry 1997; 64: 295.
- Yoo J.U., Barthel T.S., Nishimura K., et al. The chon- drogenic potential of human bone-marrow-derived mes- enchymal progenitor cells. Journal of Bone and Joint Surgery. American Volume 1998; 80: 1745.
- Sanchez-Ramos J., Song S.,Cardozo-Pelaez F., et al. Adult bone marrow stromal cells differntiate into neural cells in vitro. Experimental Neurology 2000; 164: 147.
- Min J., Sullivan M., Yand Y., et al. Significant im- provement of heart function by cotransplantation of hu- man mesenchymal stem cells and cardiomyocytes in postinfarcted pigs. The annals of thoracic surgery. 2002; 74 (5): 1568.
- Koe O., Lazarus H. Mesenchymal cells: heading into the clinic. Bone Marrow Transplantation 2001; 27: 235.
- Erices A., Conget P. and Minguell J. Mesenchymal progenitor cells in human umbilical cord blood. British Journal of Hematology 2000; 109: 235.
- Romanov Y., Svintsitskaya V., Smirnov V. Seraching for alternative sources of postnatal human mesenchymal stem cells: Candidate MSC-like cells from umbilical cord. Stem Cells 2003; 21: 105.
- Huss R., Lange C., Weissinger E., et al. Evidence of peripheral blood derived, Plastic-adherent CD34 -/ low he- matopoietic stem cell clones with Mesenchymal stem cell characteristics. Stem Cells 2000; 18. No 4: 252.
- Fernandez M., Simon V., Herrera G., et al. Detection of stromal cells in peripheral blood progenitor cell collec- tions from bleast cancer patients. Bone Marroew Trans- plantation 1997; 20: 265.
- Huss R. Isolation of Primary and immortalized CD34- Hematopoietic and Mesenchymal stem cells from various sources. Stem Cells 2000; 18:1.
- Singer JW., Charbord P., Keating A., et al. Simian virus-40 transformed adherent cells from human long- term cultures: Cloned cell lines produce cells with stro- mal and hematopoietic characteristics. Blood 1987; 70: 464.
- Huss R., Hong DS., McSweeney PA., et al. Differen- tiation of canine marrow cells with hematopoietic charac- teristics from an adherent stromal cell precursor. Proc. Natl. Acad. Sci. USA 1995; 92: 748. [Abstract]
- Huss R., Hoy CA., Deeg WJ. Stroma derived hema- topoietic progenitors: Cell cycle dependent proliferation and differentiation. In: Gluckman E, Coulombel E, and Eds. Ontogeny of Hematgopoisis: A Plastic Anemia. Col- loque Inserm. Parris: Libbey Eurotext 1995; 235: 209.
- Fernando Florenzano MD and José J. Minguell Autologous mesenchymal stem cell transplantation after acute myocardial infarction The American Journal of Cardiology Volume 95, Issue 3 , 1 February 2005, Page 435
| Files | ||
| Issue | Vol 2, No 1 (2005) | |
| Section | Articles | |
| Keywords | ||
| #No Keywords# | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
