Developing a Multichannel Bioreactor with a Collagen Scaffold, ECM, and Cryoprecipitate to Significantly Produce Platelets from Umbilical Cord Blood Stem Cells
-
Mohammad Hossein Derakhty Gonbad
,
Ali Baradar Khoshfetrat
,
Ali Akbar Movassaghpour
,
Zohre Sanaat
,
Hojjatollah Nozad Charoudeh
Abstract
Background: Platelets play a key role in the treatment of thrombocytopenia. Nowadays, platelets (PLTs) are only obtained through blood donation. However, due to the limitations in their preparation and storage, they are produced in laboratories, especially through bioreactors that convert megakaryocytes from stem cells into large-scale injectable PLTs.
Materials and Methods: In this study, the CD34 cells isolated from cord blood were differentiated into megakaryocytes. A 6-chamber bioreactor with a two-layer collagen scaffold, several ECM factors, and human cryoprecipitate were used to simulate the structure of the bone marrow. After the addition of megakaryocytes to the scaffold, PLTs were produced due to the flow pressure and the interaction between the scaffold structure and the ECM factors.
Results: CD41 + cells were expanded 100 times as much as CD34 + cord blood stem cells. The mean PLT release from one megakaryocyte in the pure collagen scaffold was 17.42 PLTs. Once fibrin, fibronectin, hyaluronic acid, and cryoprecipitates were added to collagen, the mean PLT release was 21.4, 22.4, 23.9, and 27.37, respectively. With the simultaneous addition of three factors to collagen (CFFH) and then four factors (CFFHC), the number of PLTs reached 30.52 and then 34.
Conclusion: Functional PLTs can be produced on a large scale with a multi-chamber bioreactor using a combination of ECM and cryoprecipitate with collagen scaffolding.
1. Noh JY. Megakaryopoiesis and Platelet Biology: Roles of Transcription Factors and Emerging Clinical Implications. Int J Mol Sci. 2021;22(17):9615.
2. Liu C, Huang B, Wang H, et al. The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation. Stem Cells Transl Med. 2021;10(12):1614-1620.
3. Yang J, Luan J, Shen Y, et al. Developments in the production of platelets from stem cells (Review). Mol Med Rep. 2021;23(1):7.
4. Zhang B, Wu X, Zi G, et al. Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions. Cell Prolif. 2021;54(4):e13002.
5. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
6. Luanpitpong S, Poohadsuan J, Klaihmon P, et al. Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation. Stem Cells. 2021;39(6):787-802.
7. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
8. Reems JA, Pineault N, Sun S. In vitro megakaryocyte production and platelet biogenesis: state of the art. Transfus Med Rev. 2010;24(1):33-43
9. Di Buduo CA, Soprano PM, Miguel CP, et al. A Gold Standard Protocol for Human Megakaryocyte Culture Based on the Analysis of 1,500 Umbilical Cord Blood Samples. Thromb Haemost. 2021;121(4):538-542
10. de Witt SM, Swieringa F, Cavill R, et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun. 2014;5:4257
11. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
12. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
13. Sullenbarger B, Bahng JH, Gruner R, et al. Prolonged continuous in vitro human platelet production using three-dimensional scaffolds. Exp Hematol. 2009;37(1):101-10.
14. Shepherd JH, Howard D, Waller AK, et al. Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity. Biomaterials. 2018;182:135-144.
15. Nakagawa Y, Nakamura S, Nakajima M, et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013;41(8):742-8
16. Kumon H, Sakuma S, Nakamura S, et al. Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production. Micromachines (Basel). 2021;12(10):1253.
17. Di Buduo CA, Wray LS, Tozzi L, et al. Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies. Blood. 2015;125(14):2254-64.
18. Currao M, Malara A, Di Buduo CA, et al. Hyaluronan based hydrogels provide an improved model to study megakaryocyte-matrix interactions. Exp Cell Res. 2016;346(1):1-8
19. Fujiyama S, Hori N, Sato T, et al. Development of an ex vivo xenogeneic bone environment producing human platelet-like cells. PLoS One. 2020;15(4):e0230507.
20. Yasui K, Matsumoto K, Hirayama F, et al. Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation. Stem Cells. 2003;21(2):143-51.
21. Liu SS, Zhang C, Zhang Xi, et al. Human umbilical cord blood-derived stromal cells: A new source of stromal cells in hematopoietic stem cell transplantation. Crit Rev Oncol Hematol. 2014;90(2):93-8.
22. Lagrue-Lak-Hal AH, Debili N, Kingbury G, et al. Expression and function of the collagen receptor GPVI during megakaryocyte maturation. J Biol Chem. 2001;276(18):15316-25.
23. Avanzi MP, Oluwadara OE, Cushing MM, et al. A novel bioreactor and culture method drives high yields of platelets from stem cells. Transfusion. 2016;56(1):170-8.
24. Philip J, Kumarage S, Chatterjee T, et al. The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours. Lab Med. 2014;45(2):111-5.
25. Wong H, Curry N. Cryoprecipitate transfusion: current perspectives. Int J Clin Transfus. 2016;4: 89-97.
26. Davidenko N, Schuster CF, Bax DV, et al. Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics. Acta Biomater. 2015;25:131-142.
27. Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDC-NHS Cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater. 2015;3(1):014902
28. Varotto E, Munaretto E, Stefanachi F, et al. Diagnostic challenges in acute monoblastic/monocytic leukemia in children. Front Pediatr. 2022;10:911093.
1. Noh JY. Megakaryopoiesis and Platelet Biology: Roles of Transcription Factors and Emerging Clinical Implications. Int J Mol Sci. 2021;22(17):9615.
2. Liu C, Huang B, Wang H, et al. The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation. Stem Cells Transl Med. 2021;10(12):1614-1620.
3. Yang J, Luan J, Shen Y, et al. Developments in the production of platelets from stem cells (Review). Mol Med Rep. 2021;23(1):7.
4. Zhang B, Wu X, Zi G, et al. Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions. Cell Prolif. 2021;54(4):e13002.
5. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
6. Luanpitpong S, Poohadsuan J, Klaihmon P, et al. Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation. Stem Cells. 2021;39(6):787-802.
7. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
8. Reems JA, Pineault N, Sun S. In vitro megakaryocyte production and platelet biogenesis: state of the art. Transfus Med Rev. 2010;24(1):33-43
9. Di Buduo CA, Soprano PM, Miguel CP, et al. A Gold Standard Protocol for Human Megakaryocyte Culture Based on the Analysis of 1,500 Umbilical Cord Blood Samples. Thromb Haemost. 2021;121(4):538-542
10. de Witt SM, Swieringa F, Cavill R, et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun. 2014;5:4257
11. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
12. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
13. Sullenbarger B, Bahng JH, Gruner R, et al. Prolonged continuous in vitro human platelet production using three-dimensional scaffolds. Exp Hematol. 2009;37(1):101-10.
14. Shepherd JH, Howard D, Waller AK, et al. Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity. Biomaterials. 2018;182:135-144.
15. Nakagawa Y, Nakamura S, Nakajima M, et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013;41(8):742-8
16. Kumon H, Sakuma S, Nakamura S, et al. Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production. Micromachines (Basel). 2021;12(10):1253.
17. Di Buduo CA, Wray LS, Tozzi L, et al. Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies. Blood. 2015;125(14):2254-64.
18. Currao M, Malara A, Di Buduo CA, et al. Hyaluronan based hydrogels provide an improved model to study megakaryocyte-matrix interactions. Exp Cell Res. 2016;346(1):1-8
19. Fujiyama S, Hori N, Sato T, et al. Development of an ex vivo xenogeneic bone environment producing human platelet-like cells. PLoS One. 2020;15(4):e0230507.
20. Yasui K, Matsumoto K, Hirayama F, et al. Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation. Stem Cells. 2003;21(2):143-51.
21. Liu SS, Zhang C, Zhang Xi, et al. Human umbilical cord blood-derived stromal cells: A new source of stromal cells in hematopoietic stem cell transplantation. Crit Rev Oncol Hematol. 2014;90(2):93-8.
22. Lagrue-Lak-Hal AH, Debili N, Kingbury G, et al. Expression and function of the collagen receptor GPVI during megakaryocyte maturation. J Biol Chem. 2001;276(18):15316-25.
23. Avanzi MP, Oluwadara OE, Cushing MM, et al. A novel bioreactor and culture method drives high yields of platelets from stem cells. Transfusion. 2016;56(1):170-8.
24. Philip J, Kumarage S, Chatterjee T, et al. The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours. Lab Med. 2014;45(2):111-5.
25. Wong H, Curry N. Cryoprecipitate transfusion: current perspectives. Int J Clin Transfus. 2016;4: 89-97.
26. Davidenko N, Schuster CF, Bax DV, et al. Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics. Acta Biomater. 2015;25:131-142.
27. Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDC-NHS Cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater. 2015;3(1):014902
28. Varotto E, Munaretto E, Stefanachi F, et al. Diagnostic challenges in acute monoblastic/monocytic leukemia in children. Front Pediatr. 2022;10:911093.
29. Baigger A, Blasczyk R, Figueiredo C. Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application. Transfus Med Hemother. 2017;44(3):165-173.
30. Blin A, Le Goff A, Magniez A, et al. Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics. Sci Rep. 2016;6:21700
31. Thon JN, Mazutis L, Wu S, et al. Platelet bioreactor-on-a-chip. Blood. 2014;124(12):1857-67.
32. Vučetić D, Ilić V, Vojvodić D, et al. Flow cytometry analysis of platelet populations: usefulness for monitoringthe storage lesion in pooled buffy-coat platelet concentrates. Blood Transfus. 2018;16(1):83-92.
33. Nagao Y, Masuda R, Ando A, et al. Whole Blood Platelet Aggregation Test and Prediction of Hemostatic Difficulty After Tooth Extraction in Patients Receiving Antiplatelet Therapy. Clin Appl Thromb Hemost. 2018;24(1):151-156.
34. Peerschke EIB, Castellone DD, Stroobants AK, et al. Reference range determination for whole-blood platelet aggregation using the Multiplate analyzer. Am J Clin Pathol. 2014;142(5):647-56.
35. Choi JL, Li S, Han JY. Platelet function tests: a review of progresses in clinical application. Biomed Res Int. 2014;2014:456569.
36. Pallotta I, Lovett M, Kaplan DL, et al. Three-dimensional system for the in vitro study of megakaryocytes and functional platelet production using silk-based vascular tubes. Tissue Eng Part C Methods. 2011;17(12):1223-32.
37. Saville HM, Howard D, Ghevaert C, et al. A Mathematical Model of a Valve-Controlled Bioreactor for Platelet Production. Front Mech Eng. 2022;8(858931).
38. Avanzi MP, Izak M, Oluwadara OE, et al. Actin inhibition increases megakaryocyte proplatelet formation through an apoptosis-dependent mechanism. PLoS One. 2015;10(4):e0125057.
39. Poirault-Chassac S, Nguyen KA, Pietrzyk A, et al. Terminal platelet production is regulated by von Willebrand factor. PLoS One. 2013;8(5):e63810.
40. Baigger A, Blasczyk R, Figueiredo C, et al. Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application. Transfus Med Hemother. 2017;44(3):165-173.
41. Brill A, Fuchs TA, Chauhan AK, et al. von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models. Blood. 2011;117(4):1400-7.
2. Liu C, Huang B, Wang H, et al. The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation. Stem Cells Transl Med. 2021;10(12):1614-1620.
3. Yang J, Luan J, Shen Y, et al. Developments in the production of platelets from stem cells (Review). Mol Med Rep. 2021;23(1):7.
4. Zhang B, Wu X, Zi G, et al. Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions. Cell Prolif. 2021;54(4):e13002.
5. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
6. Luanpitpong S, Poohadsuan J, Klaihmon P, et al. Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation. Stem Cells. 2021;39(6):787-802.
7. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
8. Reems JA, Pineault N, Sun S. In vitro megakaryocyte production and platelet biogenesis: state of the art. Transfus Med Rev. 2010;24(1):33-43
9. Di Buduo CA, Soprano PM, Miguel CP, et al. A Gold Standard Protocol for Human Megakaryocyte Culture Based on the Analysis of 1,500 Umbilical Cord Blood Samples. Thromb Haemost. 2021;121(4):538-542
10. de Witt SM, Swieringa F, Cavill R, et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun. 2014;5:4257
11. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
12. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
13. Sullenbarger B, Bahng JH, Gruner R, et al. Prolonged continuous in vitro human platelet production using three-dimensional scaffolds. Exp Hematol. 2009;37(1):101-10.
14. Shepherd JH, Howard D, Waller AK, et al. Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity. Biomaterials. 2018;182:135-144.
15. Nakagawa Y, Nakamura S, Nakajima M, et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013;41(8):742-8
16. Kumon H, Sakuma S, Nakamura S, et al. Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production. Micromachines (Basel). 2021;12(10):1253.
17. Di Buduo CA, Wray LS, Tozzi L, et al. Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies. Blood. 2015;125(14):2254-64.
18. Currao M, Malara A, Di Buduo CA, et al. Hyaluronan based hydrogels provide an improved model to study megakaryocyte-matrix interactions. Exp Cell Res. 2016;346(1):1-8
19. Fujiyama S, Hori N, Sato T, et al. Development of an ex vivo xenogeneic bone environment producing human platelet-like cells. PLoS One. 2020;15(4):e0230507.
20. Yasui K, Matsumoto K, Hirayama F, et al. Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation. Stem Cells. 2003;21(2):143-51.
21. Liu SS, Zhang C, Zhang Xi, et al. Human umbilical cord blood-derived stromal cells: A new source of stromal cells in hematopoietic stem cell transplantation. Crit Rev Oncol Hematol. 2014;90(2):93-8.
22. Lagrue-Lak-Hal AH, Debili N, Kingbury G, et al. Expression and function of the collagen receptor GPVI during megakaryocyte maturation. J Biol Chem. 2001;276(18):15316-25.
23. Avanzi MP, Oluwadara OE, Cushing MM, et al. A novel bioreactor and culture method drives high yields of platelets from stem cells. Transfusion. 2016;56(1):170-8.
24. Philip J, Kumarage S, Chatterjee T, et al. The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours. Lab Med. 2014;45(2):111-5.
25. Wong H, Curry N. Cryoprecipitate transfusion: current perspectives. Int J Clin Transfus. 2016;4: 89-97.
26. Davidenko N, Schuster CF, Bax DV, et al. Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics. Acta Biomater. 2015;25:131-142.
27. Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDC-NHS Cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater. 2015;3(1):014902
28. Varotto E, Munaretto E, Stefanachi F, et al. Diagnostic challenges in acute monoblastic/monocytic leukemia in children. Front Pediatr. 2022;10:911093.
1. Noh JY. Megakaryopoiesis and Platelet Biology: Roles of Transcription Factors and Emerging Clinical Implications. Int J Mol Sci. 2021;22(17):9615.
2. Liu C, Huang B, Wang H, et al. The heterogeneity of megakaryocytes and platelets and implications for ex vivo platelet generation. Stem Cells Transl Med. 2021;10(12):1614-1620.
3. Yang J, Luan J, Shen Y, et al. Developments in the production of platelets from stem cells (Review). Mol Med Rep. 2021;23(1):7.
4. Zhang B, Wu X, Zi G, et al. Large-scale generation of megakaryocytes from human embryonic stem cells using transgene-free and stepwise defined suspension culture conditions. Cell Prolif. 2021;54(4):e13002.
5. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
6. Luanpitpong S, Poohadsuan J, Klaihmon P, et al. Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation. Stem Cells. 2021;39(6):787-802.
7. Wang B, Zheng J. Platelet generation in vivo and in vitro. Springerplus. 2016;5(1):787.
8. Reems JA, Pineault N, Sun S. In vitro megakaryocyte production and platelet biogenesis: state of the art. Transfus Med Rev. 2010;24(1):33-43
9. Di Buduo CA, Soprano PM, Miguel CP, et al. A Gold Standard Protocol for Human Megakaryocyte Culture Based on the Analysis of 1,500 Umbilical Cord Blood Samples. Thromb Haemost. 2021;121(4):538-542
10. de Witt SM, Swieringa F, Cavill R, et al. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun. 2014;5:4257
11. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
12. Mazzi S, Lordier L, Debili N, et al. Megakaryocyte and polyploidization. Exp Hematol. 2018;57:1-13.
13. Sullenbarger B, Bahng JH, Gruner R, et al. Prolonged continuous in vitro human platelet production using three-dimensional scaffolds. Exp Hematol. 2009;37(1):101-10.
14. Shepherd JH, Howard D, Waller AK, et al. Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity. Biomaterials. 2018;182:135-144.
15. Nakagawa Y, Nakamura S, Nakajima M, et al. Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013;41(8):742-8
16. Kumon H, Sakuma S, Nakamura S, et al. Microfluidic Bioreactor Made of Cyclo-Olefin Polymer for Observing On-Chip Platelet Production. Micromachines (Basel). 2021;12(10):1253.
17. Di Buduo CA, Wray LS, Tozzi L, et al. Programmable 3D silk bone marrow niche for platelet generation ex vivo and modeling of megakaryopoiesis pathologies. Blood. 2015;125(14):2254-64.
18. Currao M, Malara A, Di Buduo CA, et al. Hyaluronan based hydrogels provide an improved model to study megakaryocyte-matrix interactions. Exp Cell Res. 2016;346(1):1-8
19. Fujiyama S, Hori N, Sato T, et al. Development of an ex vivo xenogeneic bone environment producing human platelet-like cells. PLoS One. 2020;15(4):e0230507.
20. Yasui K, Matsumoto K, Hirayama F, et al. Differences between peripheral blood and cord blood in the kinetics of lineage-restricted hematopoietic cells: implications for delayed platelet recovery following cord blood transplantation. Stem Cells. 2003;21(2):143-51.
21. Liu SS, Zhang C, Zhang Xi, et al. Human umbilical cord blood-derived stromal cells: A new source of stromal cells in hematopoietic stem cell transplantation. Crit Rev Oncol Hematol. 2014;90(2):93-8.
22. Lagrue-Lak-Hal AH, Debili N, Kingbury G, et al. Expression and function of the collagen receptor GPVI during megakaryocyte maturation. J Biol Chem. 2001;276(18):15316-25.
23. Avanzi MP, Oluwadara OE, Cushing MM, et al. A novel bioreactor and culture method drives high yields of platelets from stem cells. Transfusion. 2016;56(1):170-8.
24. Philip J, Kumarage S, Chatterjee T, et al. The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours. Lab Med. 2014;45(2):111-5.
25. Wong H, Curry N. Cryoprecipitate transfusion: current perspectives. Int J Clin Transfus. 2016;4: 89-97.
26. Davidenko N, Schuster CF, Bax DV, et al. Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics. Acta Biomater. 2015;25:131-142.
27. Shepherd DV, Shepherd JH, Ghose S, et al. The process of EDC-NHS Cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment. APL Mater. 2015;3(1):014902
28. Varotto E, Munaretto E, Stefanachi F, et al. Diagnostic challenges in acute monoblastic/monocytic leukemia in children. Front Pediatr. 2022;10:911093.
29. Baigger A, Blasczyk R, Figueiredo C. Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application. Transfus Med Hemother. 2017;44(3):165-173.
30. Blin A, Le Goff A, Magniez A, et al. Microfluidic model of the platelet-generating organ: beyond bone marrow biomimetics. Sci Rep. 2016;6:21700
31. Thon JN, Mazutis L, Wu S, et al. Platelet bioreactor-on-a-chip. Blood. 2014;124(12):1857-67.
32. Vučetić D, Ilić V, Vojvodić D, et al. Flow cytometry analysis of platelet populations: usefulness for monitoringthe storage lesion in pooled buffy-coat platelet concentrates. Blood Transfus. 2018;16(1):83-92.
33. Nagao Y, Masuda R, Ando A, et al. Whole Blood Platelet Aggregation Test and Prediction of Hemostatic Difficulty After Tooth Extraction in Patients Receiving Antiplatelet Therapy. Clin Appl Thromb Hemost. 2018;24(1):151-156.
34. Peerschke EIB, Castellone DD, Stroobants AK, et al. Reference range determination for whole-blood platelet aggregation using the Multiplate analyzer. Am J Clin Pathol. 2014;142(5):647-56.
35. Choi JL, Li S, Han JY. Platelet function tests: a review of progresses in clinical application. Biomed Res Int. 2014;2014:456569.
36. Pallotta I, Lovett M, Kaplan DL, et al. Three-dimensional system for the in vitro study of megakaryocytes and functional platelet production using silk-based vascular tubes. Tissue Eng Part C Methods. 2011;17(12):1223-32.
37. Saville HM, Howard D, Ghevaert C, et al. A Mathematical Model of a Valve-Controlled Bioreactor for Platelet Production. Front Mech Eng. 2022;8(858931).
38. Avanzi MP, Izak M, Oluwadara OE, et al. Actin inhibition increases megakaryocyte proplatelet formation through an apoptosis-dependent mechanism. PLoS One. 2015;10(4):e0125057.
39. Poirault-Chassac S, Nguyen KA, Pietrzyk A, et al. Terminal platelet production is regulated by von Willebrand factor. PLoS One. 2013;8(5):e63810.
40. Baigger A, Blasczyk R, Figueiredo C, et al. Towards the Manufacture of Megakaryocytes and Platelets for Clinical Application. Transfus Med Hemother. 2017;44(3):165-173.
41. Brill A, Fuchs TA, Chauhan AK, et al. von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models. Blood. 2011;117(4):1400-7.
| Files | ||
| Issue | Vol 17, No 4 (2023) | |
| Section | Original Article(s) | |
| DOI | https://doi.org/10.18502/ijhoscr.v17i4.13916 | |
| Keywords | ||
| Bioreactor; Scaffolds; Cryoprecipitate; Platelet; Umbilical cord blood stem cell | ||
| Rights and permissions | |
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
How to Cite
1.
Derakhty Gonbad MH, Baradar Khoshfetrat A, Movassaghpour AA, Sanaat Z, Nozad Charoudeh H. Developing a Multichannel Bioreactor with a Collagen Scaffold, ECM, and Cryoprecipitate to Significantly Produce Platelets from Umbilical Cord Blood Stem Cells. Int J Hematol Oncol Stem Cell Res. 2023;17(4):245-256.
