Therapeutic Potential and Comparison of the Effect of Mummy on Mesenchymal Stem Cells Derived from Wharton's Jelly and Adipose Cultured with Human Fibroblast
Abstract
Background: The Wound healing process, as a coordinated physiological mechanism, is a critical subject in medicine. The slow healing and scar formation associated with numerous conventional therapies have led researchers to seek new and more effective therapeutics. This study evaluated the effects of Mummy material, Wharton Jelly Stem Cells (WJSCs), and Adipocyte Stem cells (ASCs) on the fibroblast migration and proliferation.
Materials and Methods: It was demonstrated that fibroblast cells could attach to three-dimensional (3D) scaffolds in the mentioned microenvironment. ASCs and WJSCs were enriched from human adipose tissue and women undergoing cesarean section, respectively. The proliferation rate, migration, expression of fibronectin, collagen I, III, and cell adhesion on PCL scaffold in the presence of mummy material were investigated.
Results: The results emphasized the importance of Mummy material, ASCs, and WJSCs in the migration and proliferation of fibroblast cells. The presence of the aforementioned components and cells enhanced the expression of fibronectin (FN1) and collagen types I and III. Additionally, the mummy material was found to promote the proliferation of ADSCs and WJSCs seeded on the PCL scaffold. Together, these findings demonstrate a valuable in vitro technique for studying the healing process.
Conclusion: As a result, the potential for using Mummy material and stem cell-based therapeutics in wound healing is exciting.
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9. Jonidi Shariatzadeh F, Currie S, Logsetty S, et al. Enhancing wound healing and minimizing scarring: A comprehensive review of nanofiber technology in wound dressings. Prog Mater Sci. 2025; 147:101350.
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11. Cialdai F, Risaliti C, Monici M. Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Front Bioeng Biotechnol. 2022:10:958381.
12. Lopatina T, Favaro E, Grange C, et al. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia. Sci Rep. 2018. 8(1): 17458.
13. Rafat A, Mohammadi Roushandeh A, Alizadeh A, et al. Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells. Cell J. 2019. 20(4): 450-458.
14. Duscher D, Barrera J, Wong VW, et al. Stem cells in wound healing: the future of regenerative medicine? A Mini-Review. Gerontology. 2016. 62(2): 216-25.
15. Hasanpour Khodaei S, Roshangar L, Sebet kam SH, et al. The Effect of Mummy Substance on Matrix Protein Synthesis by Human Adipose-Derived Stem Cells and Dermal Fibroblast and Their Behavior on Plated PCL Scaffold. Crescent J Med Biol Sci. 2021; 8(3):198-204.
16. Lee DE, Ayoub N, Agrawal DK. Mesenchymal stem cells and cutaneous wound healing: novel methods to increase cell delivery and therapeutic efficacy. Stem Cell Res Ther. 2016. 7: 37.
17. Arno AI, Amini-Nik S, Blit PH, et al. Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling. Stem Cell Res Ther. 2014. 5(1): 28.
18. Dehghan M, Faradonbeh AS. The effect of mummy on the healing of bone fractures. Afr J Pharm Pharmacol. 2012; 6(5): 305-309.
19. Heipertz EL, Zynda ER, Stav-Noraas TE, et al. Current Perspectives on “Off-The-Shelf” Allogeneic NK and CAR-NK Cell Therapies. Front Immunol. 2021:12:732135.
20. Abshenas J, Kheirandish R, Salary AR. Gastroprotective effect of mummy on induced gastric ulcer in rats. Comp Clin Path. 2014; 23(2): 305-309.
21. Su WT, Wu PS, Huang TY. Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly (ε-caprolactone) nanofibers containing strontium phosphate. Mater Sci Eng C Mater Biol Appl. 2015; 46:427-34.
22. BalekarN, Katkam NG, Nakpheng T, et al. Evaluation of the wound healing potential of Wedelia trilobata (L.) leaves. J Ethnopharmacol. 2012; 141(3):817-24.
23. Morrovati F, Karimian Fathi N, Soleimani Rad J, et al. Mummy Prevents IL-1β-Induced Inflammatory Responses and Cartilage Matrix Degradation via Inhibition of NF-қB Subunits Gene Expression in Pellet Culture System. Adv Pharm Bull. 2018; 8(2): 283-289.
24. Heydarkhan-Hagvall S, Schenke-Layland K, Dhanasopon AP, et al. Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering. Biomaterials. 2008; 29(19): 2907-14.
25. Nourian Dehkordi A, Mirahmadi Babaheydari F , Chehelgerdi M, et al. Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies. Stem Cell Res Ther. 2019; 10(1):111.
26. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014:4:177.
27. Willerth SM, Sakiyama-Elbert SE. Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery. Stem J. 2019;1(1):1-25.
28. Hazrati R, Davaran S, Omidi Y. Bioactive functional scaffolds for stem cells delivery in wound healing and skin regeneration. React Funct Polym. 2022; 174: 105233.
29. Mazini L, Ezzoubi M, Malka G. Overview of current adipose-derived stem cell (ADSCs) processing involved in therapeutic advancements: flow chart and regulation updates before and after COVID-19. Stem Cell Res Ther. 2021;12(1):1.
30. Hassanpour M, Cheraghi O, Siavashi V, et al. A reversal of age-dependent proliferative capacity of endothelial progenitor cells from different species origin in in vitro condition. J Cardiovasc Thorac Res. 2016; 8(3):102-106.
31. Mankuzhy PD, Ramesh ST, Thirupathi Y, et al. The preclinical and clinical implications of fetal adnexa derived mesenchymal stromal cells in wound healing therapy. Wound Repair Regen. 2021; 29(3):347-369.
32. Khodaie SH, Roshangar L, Sabetkam SH, et al. Impact of mummy substance on the proliferation and migration of human adipose-derived stem cells and fibroblasts in separate or co-culture model. Indian J Pharm Sci. 2018. 80(3): 516-524.
33. Sadeghi SMH, Hosseini Khameneh SM, Khodadoost M, et al. Efficacy of Momiai in Tibia Fracture Repair: A Randomized Double-Blinded Placebo-Controlled Clinical Trial. J Altern Complement Med. 2020; 26(6): 521-528.
34. Khodaie SH, Roshangar L, Sabetkam S, et al. Impact of Mummy Substance on the Proliferation and Migration of Human Adipose-derived Stem Cells and Fibroblasts in Separate or Co-culture Model. Indian J Pharm Sci. 2018; 80(3):516-524.
35. Liu X, Wang Z, Wang R, et al. Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int J Mol Med. 2013; 31(2):407-15.
36. Arno AI, Amini-Nik S, Blit PH, et al. Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling. Stem Cell Res Ther. 2014; 5(1):28.
37. Jeon YK, Jang YH, Yoo DR, et al. Mesenchymal stem cells' interaction with skin: Wound-healing effect on fibroblast cells and skin tissue. Wound Repair Regen. 2010; 18(6):655-61.
38. Wang L, Li ZH, Li Y, et al. Therapeutic applications of exosomes from adipose-derived stem cells in antifibrosis. CJPRS. 2021; 3(3): 61-166.
39. Nie C, Yang D, Xu J, et al. Locally administered adipose-derived stem cells accelerate wound healing through differentiation and vasculogenesis. Cell Transplant. 2011; 20(2): 205-16.
40. Zhang B, Gao L, Ma L, et al. 3D Bioprinting: A Novel Avenue for Manufacturing Tissues and Organs. Engineering. 2019;5(4): 777-794.
41. Mohammad Ghorbani F, Kaffashi M, Shokrollahi P, et al. PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation. Carbohydr Polym. 2015; 118: 133-142.
2. Tottoli EM, Dorati R, Genta I, et al. Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration. Pharmaceutics, 2020. 12(8): 735.
3. Ayavoo T, Murugesan K, Gnanasekaran A. Roles and mechanisms of stem cell in wound healing. Stem Cell Investig. 2021; 8:4.
4. Słonimska P, Sachadyn P , Zieliński J, et al. Chemotherapy-Mediated Complications of Wound Healing: An Understudied Side Effect. Adv Wound Care (New Rochelle), 2024. 13(4): 187-199.
5. Chakraverty R, Teshima T. Graft-versus-host disease: a disorder of tissue regeneration and repair. Blood. 2021; 138(18): 1657-1665.
6. García-Sánchez D, Fernández D, Rodríguez-Rey JC, et al. Enhancing survival, engraftment, and osteogenic potential of mesenchymal stem cells. World J Stem Cells, 2019. 11(10): 748-763.
7. Morett L, Stalfort J, Barker TH, et al. The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation. J Biol Chem. 2022; 298(2):101530.
8. Rafat A, Bahmanzadeh M, Soleimani Asl S, et al. The role of melatonin preconditioning on survival of bone marrow-derived mesenchymal stem cells in differentiation to osteoblasts. Iran Red Crescent Med J. 2018; 20(11):0-0.
9. Jonidi Shariatzadeh F, Currie S, Logsetty S, et al. Enhancing wound healing and minimizing scarring: A comprehensive review of nanofiber technology in wound dressings. Prog Mater Sci. 2025; 147:101350.
10. Amirrah IN, Lokanathan Y, Zulkiflee I, et al. A Comprehensive Review on Collagen Type I Development of Biomaterials for Tissue Engineering: From Biosynthesis to Bioscaffold. Biomedicines. 2022; 10(9):2307.
11. Cialdai F, Risaliti C, Monici M. Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Front Bioeng Biotechnol. 2022:10:958381.
12. Lopatina T, Favaro E, Grange C, et al. PDGF enhances the protective effect of adipose stem cell-derived extracellular vesicles in a model of acute hindlimb ischemia. Sci Rep. 2018. 8(1): 17458.
13. Rafat A, Mohammadi Roushandeh A, Alizadeh A, et al. Comparison of The Melatonin Preconditioning Efficacy between Bone Marrow and Adipose-Derived Mesenchymal Stem Cells. Cell J. 2019. 20(4): 450-458.
14. Duscher D, Barrera J, Wong VW, et al. Stem cells in wound healing: the future of regenerative medicine? A Mini-Review. Gerontology. 2016. 62(2): 216-25.
15. Hasanpour Khodaei S, Roshangar L, Sebet kam SH, et al. The Effect of Mummy Substance on Matrix Protein Synthesis by Human Adipose-Derived Stem Cells and Dermal Fibroblast and Their Behavior on Plated PCL Scaffold. Crescent J Med Biol Sci. 2021; 8(3):198-204.
16. Lee DE, Ayoub N, Agrawal DK. Mesenchymal stem cells and cutaneous wound healing: novel methods to increase cell delivery and therapeutic efficacy. Stem Cell Res Ther. 2016. 7: 37.
17. Arno AI, Amini-Nik S, Blit PH, et al. Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling. Stem Cell Res Ther. 2014. 5(1): 28.
18. Dehghan M, Faradonbeh AS. The effect of mummy on the healing of bone fractures. Afr J Pharm Pharmacol. 2012; 6(5): 305-309.
19. Heipertz EL, Zynda ER, Stav-Noraas TE, et al. Current Perspectives on “Off-The-Shelf” Allogeneic NK and CAR-NK Cell Therapies. Front Immunol. 2021:12:732135.
20. Abshenas J, Kheirandish R, Salary AR. Gastroprotective effect of mummy on induced gastric ulcer in rats. Comp Clin Path. 2014; 23(2): 305-309.
21. Su WT, Wu PS, Huang TY. Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly (ε-caprolactone) nanofibers containing strontium phosphate. Mater Sci Eng C Mater Biol Appl. 2015; 46:427-34.
22. BalekarN, Katkam NG, Nakpheng T, et al. Evaluation of the wound healing potential of Wedelia trilobata (L.) leaves. J Ethnopharmacol. 2012; 141(3):817-24.
23. Morrovati F, Karimian Fathi N, Soleimani Rad J, et al. Mummy Prevents IL-1β-Induced Inflammatory Responses and Cartilage Matrix Degradation via Inhibition of NF-қB Subunits Gene Expression in Pellet Culture System. Adv Pharm Bull. 2018; 8(2): 283-289.
24. Heydarkhan-Hagvall S, Schenke-Layland K, Dhanasopon AP, et al. Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering. Biomaterials. 2008; 29(19): 2907-14.
25. Nourian Dehkordi A, Mirahmadi Babaheydari F , Chehelgerdi M, et al. Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies. Stem Cell Res Ther. 2019; 10(1):111.
26. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014:4:177.
27. Willerth SM, Sakiyama-Elbert SE. Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery. Stem J. 2019;1(1):1-25.
28. Hazrati R, Davaran S, Omidi Y. Bioactive functional scaffolds for stem cells delivery in wound healing and skin regeneration. React Funct Polym. 2022; 174: 105233.
29. Mazini L, Ezzoubi M, Malka G. Overview of current adipose-derived stem cell (ADSCs) processing involved in therapeutic advancements: flow chart and regulation updates before and after COVID-19. Stem Cell Res Ther. 2021;12(1):1.
30. Hassanpour M, Cheraghi O, Siavashi V, et al. A reversal of age-dependent proliferative capacity of endothelial progenitor cells from different species origin in in vitro condition. J Cardiovasc Thorac Res. 2016; 8(3):102-106.
31. Mankuzhy PD, Ramesh ST, Thirupathi Y, et al. The preclinical and clinical implications of fetal adnexa derived mesenchymal stromal cells in wound healing therapy. Wound Repair Regen. 2021; 29(3):347-369.
32. Khodaie SH, Roshangar L, Sabetkam SH, et al. Impact of mummy substance on the proliferation and migration of human adipose-derived stem cells and fibroblasts in separate or co-culture model. Indian J Pharm Sci. 2018. 80(3): 516-524.
33. Sadeghi SMH, Hosseini Khameneh SM, Khodadoost M, et al. Efficacy of Momiai in Tibia Fracture Repair: A Randomized Double-Blinded Placebo-Controlled Clinical Trial. J Altern Complement Med. 2020; 26(6): 521-528.
34. Khodaie SH, Roshangar L, Sabetkam S, et al. Impact of Mummy Substance on the Proliferation and Migration of Human Adipose-derived Stem Cells and Fibroblasts in Separate or Co-culture Model. Indian J Pharm Sci. 2018; 80(3):516-524.
35. Liu X, Wang Z, Wang R, et al. Direct comparison of the potency of human mesenchymal stem cells derived from amnion tissue, bone marrow and adipose tissue at inducing dermal fibroblast responses to cutaneous wounds. Int J Mol Med. 2013; 31(2):407-15.
36. Arno AI, Amini-Nik S, Blit PH, et al. Human Wharton’s jelly mesenchymal stem cells promote skin wound healing through paracrine signaling. Stem Cell Res Ther. 2014; 5(1):28.
37. Jeon YK, Jang YH, Yoo DR, et al. Mesenchymal stem cells' interaction with skin: Wound-healing effect on fibroblast cells and skin tissue. Wound Repair Regen. 2010; 18(6):655-61.
38. Wang L, Li ZH, Li Y, et al. Therapeutic applications of exosomes from adipose-derived stem cells in antifibrosis. CJPRS. 2021; 3(3): 61-166.
39. Nie C, Yang D, Xu J, et al. Locally administered adipose-derived stem cells accelerate wound healing through differentiation and vasculogenesis. Cell Transplant. 2011; 20(2): 205-16.
40. Zhang B, Gao L, Ma L, et al. 3D Bioprinting: A Novel Avenue for Manufacturing Tissues and Organs. Engineering. 2019;5(4): 777-794.
41. Mohammad Ghorbani F, Kaffashi M, Shokrollahi P, et al. PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation. Carbohydr Polym. 2015; 118: 133-142.
| Files | ||
| Issue | Vol 19 No 3 (2025) | |
| Section | Original Article(s) | |
| Keywords | ||
| Wound healing; Adipocyte stem cells; Wharton jelly stem cells; Mummy material | ||
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How to Cite
1.
Hassanpour Khodaei S, Roshangar L, Soleimani Rad J, Sabetkam S. Therapeutic Potential and Comparison of the Effect of Mummy on Mesenchymal Stem Cells Derived from Wharton’s Jelly and Adipose Cultured with Human Fibroblast. Int J Hematol Oncol Stem Cell Res. 2025;19(3):198-209.
