Defining Permissible Time Lapse between Umbilical Cord Tissue Collection and Commencement of Cell Isolation
Abstract
Background: Umbilical cord tissue is a very rich source of mesenchymal stem cells. Instead of discarding this source we are banking the tissue along with cord blood for possible future cell based applications. The cord tissue needs to be transported and stored properly in order for it to be good enough for cell isolation at a later date. In this paper we have carried out a validation study to determine the maximum permissible time between cord tissue collection and beginning of cell culture process under defined conditions of temperature and collection media.
Methods: Ten cord tissue samples were used for this study. The umbilical cord tissue segments were transported and stored at 2 – 8oC for varying periods of time viz. 04, 11, 22 and 30 days in a defined medium after which MSCs were isolated and characterized by flow cytometry. Karyotypic studies were also performed on the isolated cells at the above time points.
Results: MSCs could be successfully isolated from 09 even samples after a storage period of 22 days and from 07 samples after a period of 30 days from the date of collection. There was no change in the morphology, immunophenotye, karyotypye and growth potential of the cells isolated from cord tissue after the maximum storage period of 30 days.
Conclusion: The umbilical cord tissue is stable for as long as 22 days if stored at the recommended storage conditions of 2 – 8oC in the defined medium.
Bruder S, Jaiswal N, and Haynesworth S. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. Journal of Cellular Biochemistry 1997; 64(2): 278–294.
Haynesworth S, Goshima J, Goldberg V and Caplan A. Characterization of cells with osteogenic potential from human marrow. Bone 1992;13(1): 81–88.
Pittenger M, Mackay M, Beck C. et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284(5411):143–147.
Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue. Stem Cells 2006; 24: 1294–1301.
McElreavey D, Irvine I, Ennis T and McLean H. Isolation, culture and characterization of fibroblast-like cells derived from the Wharton’s jelly portion of human umbilical cord. Biochem Soc Trans 1991; 19: 29(S).
Shetty P, Cooper K, Viswanathan C. Comparison of proliferative and multileneage differentiation potential of cord matrix, cord blood and bone marrow mesenchymal stem cells. Asian Journal of Transfusion Science. 2001; 1: 14 – 24.
Weiss M and Troyer D. Stem cells in the umbilical cord. Stem Cell Reviews 2006; 2: 155-162.
Can A and Karahuseyinoglu S. Human umbilical cord stroma with regard to the source of fetus derived stem cells. Stem Cells 2007; 25: 2886-2895.
Cooper K, SenMajumdar A and Viswanathan C. Derivation, expansion and characterization of clinical grade mesenchymal stem cells from umbilical cord matrix using cord blood serum. International Journal of Stem Cells. 2010; 3 : 119-128.
Shetty P, Thakur A and Viswanathan C. Dopaminergic cells, derived from a high efficiency differentiation protocol from umbilical cord derived mesenchymal stem cells alleviate symptoms in a Parkinson's disease rodent model. Cell Biol Int. 2013;37: 167–180.
Kim J, Kim S, Park S, Kim Y, Kim M, Lee M, Ryu H. Mesenchymal progenitor cells in the human umbilical cord. Ann Hematol 2004; 83: 733-738.
Covas D, Siufi J, Silva A, Orellana M. Isolation and culture of umbilical vein mesenchymal stem cells. Braz JMed Biol Res 2003; 36:1179–1183.
Sarugaser R, Lickorish D, Baksh D, Hosseini M, Davies J. Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors. Stem Cells 2005;23: 220–229.
Weiss M, Troyer D. Stem cells in the umbilical cord. Stem Cell Rev 2006; 2:155–1663
Secco M, Zucconi E, Vieira N, Fogac¸a L, Cerqueira A, Carvalho M, Jazedje T, Okamoto O, Muotri A, Zatz M. Multipotent stem cells from umbilical cord: cord is richer than blood! Stem Cells 2008; 26:146–150.
Ishige I, Nagamura-Inoue T, Honda MJ, Harnprasopwat R, Kido M, Sugimoto M, Nakauchi H, Tojo A. Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton’s jelly explants of human umbilical cord. Int J Hematol 2009;90:261–269
Kita K, Gauglitz G, Phan T, Herndon N, Jeschke G. Isolation and characterization of mesenchymal stem cells from the sub-amniotic human umbilical cord lining membrane. Stem Cells Dev 2010; 9(4):491–502.
Romanov Y, Svintsitskaya V, Smirnov V. Searching for Alternative Sources of Postnatal Human Mesenchymal Stem Cells: Candidate MSC-Like Cells from Umbilical Cord. Stem Cells 2003; 21: 105-110.
Kadivar M, Khatami S, Mortazavi Y Shokrgozar M, Taghikhani M, Soleimani M. In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells. Biochemical and Biophysical Research Communications. 2006; 340: 639–647.
Wang H, Hung S, Peng S et al. Mesenchymal Stem Cells in the Wharton’s Jelly of the Human Umbilical Cord. Stem Cells 2004; 22: 1330-1337.
Christodoulou I, Kolisis F, Papaevangeliou D, and Zoumpourlis V. Comparative evaluation of human mesenchymal stem cells of fetal (Wharton’s Jelly) and Adult (Adipose Tissue) origin during prolonged in vitro expansion: consideration for cytotherapy. Stem Cell Int. 2013; doi: 10.1155/2013/246134.
Gartner A, Pereira T, Gomes R, Luis Lucia A, Franca M, Geuna S, Armada-da-Silva and Mauricio A. Mesenchymal Stem Cells from Extra-Embryonic Tissues for Tissue Engineering – Regeneration of the Peripheral Nerve. In: Prof. Rosario Pignatello editor, Advances in Biomaterials Science and Biomedical Applications. ISBN 978-953-51-1051-4.
| Files | ||
| Issue | Vol 7, No 4 (2013) | |
| Section | Articles | |
| Keywords | ||
| Adult stem cells Mesenchymal stem cells Transportation Umbilical cord tissue | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
