Expression Analysis of Long Non-coding PCAT-1
Abstract
Background: The prostate cancer-associated non-coding RNA transcript 1 (PCAT-1) is a newly identified long non- coding RNA whose participation in tumorigenesis of a variety of cancers has been observed. In the present study, we aimed at analysis of its expression in breast cancer patients.
Subjects and Methods: The expression of PCAT-1 was assessed using real-time reverse transcription polymerase chain reaction in tumor samples obtained from 47newly diagnosed breast cancer patients as well as their corresponding adjacent non-cancerous tissues (ANCTs).
Results: We detected significant over-expression of PCAT-1 in 12/47 (25.5%) of tumoral tissues compared with their corresponding ANCTs. However, no significant association has been found between the levels of PCAT-1 transcripts and patients’ clinical data such as tumor size, stage, grade, estrogen and progesterone receptors or Her2/neu status.
Conclusion: PCAT-1 is possibly involved in the pathogenesis of fraction of breast cancers. Future studies are needed to evaluate its precise function in breast cancer.
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2015. CA Cancer J Clin. 2015;65(1):5-29.
Waldemarson S, Kurbasic E, Krogh M, et al. Proteomic analysis of breast tumors confirms the mRNA intrinsic molecular subtypes using different classifiers: a large-scale analysis of fresh frozen tissue samples. Breast Cancer Res. 2016;18(1):69.
Dianatpour M, Mehdipour P, Nayernia K, et al. Expression of Testis Specific Genes TSGA10, TEX101 and ODF3 in Breast Cancer. Iranian Red Crescent medical journal. 2012;14(11):722-6.
Kazemi-Oula G, Ghafouri-Fard S, Mobasheri MB, et al. Upregulation of RHOXF2 and ODF4 Expression in Breast Cancer Tissues. Cell journal. 2015 Fall;17(3):471-7.
Derrien T, Johnson R, Bussotti G, et al. The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression. Genome Res. 2012;22(9):1775-89.
Nikpayam E, Tasharrofi B, Sarrafzadeh S, et al. The Role of Long Non-Coding RNAs in Ovarian Cancer. Iran Biomed J. 2017;21(1):3-15.
Soudyab M, Iranpour M, Ghafouri-Fard S. The Role of Long Non-Coding RNAs in Breast Cancer. Archives of Iranian medicine. 2016;19(7):508-17.
Iranpour M, Soudyab M, Geranpayeh L, et al. Expression analysis of four long noncoding RNAs in breast cancer. Tumour Biol. 2016;37(3):2933-40.
Tasharrofi B, Soudyab M, Nikpayam E, et al. Comparative expression analysis of hypoxia-inducible factor-alpha and its natural occurring antisense in breast cancer tissues and adjacent noncancerous tissues. Cell Biochem Function. 2016;34(8):572-578.
Guo HY, Ahmed M, Zhang F, et al. Modulation of long noncoding RNAs by risk SNPs underlying genetic predispositions to prostate cancer. Nat Genet. 2016;48(10):1142-50.
Prensner JR, Iyer MK, Balbin OA, et al. Transcriptome sequencing identifies PCAT-1, a novel lincRNA implicated in prostate cancer progression. Nat Biotechnol. 2011;29(8):742.
Proverbs-Singh T, Feldman JL, Morris MJ, et al. Targeting the androgen receptor in prostate and breast cancer: several new agents in development. Endocr-Relat Cancer. 2015;22(3):R87-R106.
Chia K, O'Brien M, Brown M, et al. Targeting the Androgen Receptor in Breast Cancer. Curr Oncol Rep. 2015;17(2).
Prensner JR, Chen W, Han SM, et al. The Long Non-Coding RNA PCAT-1 Promotes Prostate Cancer Cell Proliferation through cMyc. Neoplasia. 2014;16(11):900-8.
Ye J, Coulouris G, Zaretskaya I, et al. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC bioinformatics. 2012;13:134.
Ge X, Chen Y, Liao X, et al. Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer. Med Ooncol. 2013;30(2):588.
Yan TH, Yang H, Jiang JH, et al. Prognostic significance of long non-coding RNA PCAT-1 expression in human hepatocellular carcinoma. Int J Clin Exp Pathol. 2015;8(4):4126-31.
Zhao B, Hou X, Zhan H. Long non-coding RNA PCAT-1 over-expression promotes proliferation and metastasis in non-small cell lung cancer cells. Int J Clin Exp Pathol. 2015;8(10):18482-7.
| Files | ||
| Issue | Vol 11, No 3 (2017) | |
| Section | Original Article(s) | |
| Keywords | ||
| Breast cancer lncRNA PCAT1 | ||
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