PCR-RFLP Is a Useful Tool to Distinguish between C. Dubliniensis and C. Albicans in Cancer Patients in Iran

Abstract

Introduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly.
Materials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done.
Results: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans.  The results were confirmed by sequencing and RAPD-PCR.
Conclusion:PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis.