Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition

Abstract

Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.